Figure 3
Correlation between MHC II downregulation and invasion capacity, cytotoxicity or ROS production. Correlation between Salmonella induced MHC II downregulation (X-axis) and (A) Salmonella invasion capacity, (B) macrophage viability or (C) ROS production (on the respective Y-axes) with r = Pearson correlation coefficient. MHC II downregulation capacity is presented as the average mean FL-5 signal ratios between infected and uninfected porcine alveolar macrophages (PAMinf and PAMuninf, respectively). Salmonella invasion capacity is expressed as the number of macrophages infected by a particular Salmonella strain relative to the macrophages infected by strain 112910a. Viability is expressed as the average percentage of viable cells at 24 h post inoculation relative to viable uninfected macrophages (negative control). ROS production of infected macrophages was expressed relative to the ROS production of phorbol 12-myristate 13-acetate (PMA) treated macrophages (positive control). ROS = reactive oxygen species; PAM = porcine alveolar macrophages