Figure 4

CsA does not affect interferon-stimulated gene responses and NF-AT activities. (A) Control vector pGL3 plasmid for normalization and ISRE-luciferase plasmid were transfected into fcwf-4 cells which were then incubated with or without indicated concentrations of CsA and FK506. Culture medium also contained interferon α (10 U/mL). Dual luciferase activities were measured at 48 h after transfection. (B) Plasmid vectors pGL4.30 [luc2P/NFAT-RE/Hygro] and pRL-SV40 for NF-AT response assays were transfected into fcwf-4 cells that were then cultured with or without indicated concentrations of CsA and FK506. Dual luciferase activities were measured at 48 h after transfection. Transfection efficiency was normalized by Renilla luciferase gene expression.