Figure 1

Effects of exogenous NS1 proteins on Akt phosphorylation in different cells. Hela (A, B, C) or A549 cells (D) were serum-starved for 12 h and transfected with different NS1-expressing plasmids or PNF empty vector. At 24-h post transfection, cells were lysed and subjected to Western blot analysis using the specific antibodies for phospho-Akt(Ser473), total-Akt, β-actin, or NS1.