Characterization of C-strain “Riems” TAV-epitope escape variants obtained through selective antibody pressure in cell culture

Table 1 Stability analysis of the E2 TAV escape variants D9, B5/2, Q7, S10, and O11 using partial sequencing of viral proteins E1, E2, and E ^RNS (numbers indicate nucleotide positions differing from the parental C-strain “Riems”)

Escape variant	D9 3^rd passage	B5/2 6^thpassage	S10-4^thpassage	011-5^thpassage	Q7-6^thpassage	Q7-14^thpassage	S10-18^thpassage	O11-17^thpassage	Resulting amino acid exchange C-strain to escape variant
Viral protein
E ^RNS	1649	1649	1649	1649	1649	1649	1649	1649	Isoleucin to Valine
E1	-	-	2099	2099	2099	2099	2099	2099	Tyrosine to Histidine
		2122	2122	2122	2122	2122	2122	2122	Aspartic acid to Glutamic acid
E2	-	-	2862	2862	2862	2862	2862	2862	Alanine to Valine
	2870	2870	2870	2870	2870	2870	2870	2870	Proline to Serine
	-	2889	2889	2889	2889	2889	2889	2889	Glutamic acid to Glycine

ISSN: 1297-9716