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Table 1 Comparison of fold-changes in gene expression in the infected MDM relative to the non-infected control MDM based on microarray and real time qRT-PCR results

From: Pan-genomic analysis of bovine monocyte-derived macrophage gene expression in response to in vitro infection with Mycobacterium avium subspecies paratuberculosis

Gene symbol Gene name Gene description 2 h mean fold-changes in gene expression 6 h mean fold-changes in gene expression 24 h mean fold-changes in gene expression
Microarray data Real time qRT-PCR P-value (real time qRT-PCR) Microarray data Real time qRT-PCR P-value (real time qRT-PCR) Microarray data Real time qRT-PCR P-value (real time qRT-PCR)
AREGB Amphiregulin B A growth-modulating glycoprotein NS - 1.29 ≤ 0.01 - 2.03 - 2.58 ≤ 0.05 NS - 1.54 ≤ 0.01
CCL4 Chemokine (C-C motif) ligand 4 A proinflammatory and chemotactic chemokine + 6.59 + 28.87 ≤ 0.001 + 3.11 + 9.67 ≤ 0.05 NS + 1.28 NS
CCL5 Chemokine (C-C motif) ligand 5 A proinflammatory chemokine involved in the chemotaxis of monocytes and T-helper cells + 3.56 + 5.07 ≤ 0.05 + 4.02 + 4.60 ≤ 0.05 NS + 2.11 ≤ 0.05
CCL20 Chemokine (C-C motif) ligand 20 A chemokine involved in the chemoattraction of lymphocytes and neutrophils + 10.47 + 63.19 ≤ 0.001 + 2.80 + 10.82 ≤ 0.05 NS + 1.22 NS
CD40 CD40 molecule, TNF receptor superfamily member 5 A member of the TNF-receptor superfamily. Mediates the immune and inflammatory responses + 4.44 + 6.55 ≤ 0.001 + 5.23 + 10.80 ≤ 0.001 + 1.71 + 1.82 ≤ 0.05
CFB Complement factor B A component of the alternative pathway of complement activation + 3.54 + 4.61 ≤ 0.05 + 4.84 + 8.64 ≤ 0.01 + 2.98 + 4.19 ≤ 0.001
CXCL2 Chemokine (C-X-C motif) ligand 2 An immunoregulatory chemokine produced by activated monocytes and neutrophils at sites of inflammation + 14.34 + 28.56 ≤ 0.001 + 2.38 + 3.07 ≤ 0.01 NS + 1.45 NS
IL1B Interleukin 1, beta A cytokine that mediates the inflammatory response including cell proliferation, differentiation and apoptosis + 14.41 + 48.53 ≤ 0.001 + 2.97 + 6.16 ≤ 0.05 NS + 2.25 ≤ 0.05
IL6 Interleukin 6 A cytokine that functions in inflammation and the maturation of B cells + 6.25 + 21.12 ≤ 0.001 NS + 2.51 ≤ 0.01 NS + 1.38 NS
IL15 Interleukin 15 A cytokine that regulates T and natural killer cell activation and proliferation + 3.51 + 4.40 ≤ 0.001 + 2.03 + 2.73 ≤ 0.01 NS 1.04 NS
IRF1 Interferon regulatory factor 1 A member of the interferon regulatory transcription factor family. An activator of interferon alpha and beta transcription + 6.57 + 12.64 ≤ 0.001 + 3.60 + 5.20 ≤ 0.01 NS + 1.60 ≤ 0.01
NFKB2 Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100) A pleiotropic transcription factor involved in inflammation, immunity, differentiation, cell growth and apoptosis + 3.41 + 5.37 ≤ 0.001 + 3.69 + 5.82 ≤ 0.001 NS + 1.34 NS
PIK3IP1 Phosphoinositide-3-kinase interacting protein 1 Suppresses the activity of phosphatidylinositol-3-kinase (PI3K), a regulator of cell division - 1.70 - 2.34 ≤ 0.05 NS - 1.36 NS NS + 1.54 NS
SPRY2 Sprouty homolog 2 (Drosophila) An inhibitor of receptor tyrosine kinase signalling proteins - 2.07 - 2.20 NS NS - 1.12 NS NS - 1.46 NS
TNF Tumor necrosis factor (TNF superfamily, member 2) A proinflammatory cytokine (secreted by macrophages) involved in the regulation cell proliferation, differentiation and apoptosis, and coagulation. + 13.89 + 33.01 ≤ 0.001 + 2.73 + 6.51 ≤ 0.01 NS + 1.61 ≤ 0.01
  1. Geometric mean fold-changes of gene expression (infected MDM relative to non-infected control MDM from the same animal at each time point) are given for the microarray and real time qRT-PCR data at each time point. “NS” indicates that the gene was not significantly differentially expressed at the appropriate time point. The P-values obtained from analysis of the real time qRT-PCR analysis are presented. Geometric fold-changes given for the microarray data were significantly differentially expressed using an adjusted P-value ≤ 0.10. Descriptions of the function of each gene were obtained from the Entrez Gene database [44] and the GeneCards version 3 database [45].