Repertoire of Escherichia coli agonists sensed by innate immunity receptors of the bovine udder and mammary epithelial cells

Table 1 List of primers used in this study

Gene	Primer ID	Primer sequence	Size of amplicon	Annealing temperature (°C)
18S	18sF	CGGGGAGGTAGTGACGAAA	196 bp	62
	18sR	CCGCTCCCAAGATCCAACTA
ACTB	ACTB f	ACGGGCAGGTCATCACCATC	166 bp	64
	ACTB r	AGCACCGTGTTGGCGTAGAG
PPIA	PPIA Fq	TCCGGGATTTATGTGCCAGGG	206 bp	66
	PPIA Rq	GCTTGCCATCCAACCACTCAG
TLR1	TLR1 Fq	ACCCTACTCTGAACCTCAAG	142 bp	62
	TLR1 Rq	GACTGCACACTGGATTTCTG
TLR2	TLR2 Fq	ACTGGGTGGAGAACCTCATGGTCC	307 bp	62
	TLR2 Rq	ATCTTCCGCAGCTTACAGAAGC
TLR4	TLR4 F2q	GCATGGAGCTGAATCTCTAC	238 bp	62
	TLR4 R2q	CAGGCTAAACTCTGGATAGG
TLR5	TLR5 Fq	TTCCTGCAACCTCACCCAAG	192 bp	62
	TLR5 Rq	CTGAGATTGGGCAGGTTTCG
TLR6	TLR6 Fq	CTCCGGGAGATAGTCACTTC	297 bp	62
	TLR6 Rq	GGCCCTGGATTCTATTATGG
NOD1	NOD1 F2q	TGGTCACTCACATCCGAAAC	218 bp	62
	NOD1 F2q	AGGCCTGAGATCCACATAAG
NOD2	NOD2-f	CCCAGGGGCTCAGAACTAACA	238 bp	62
	NOD2-r	CCTTCATCCTGGACGTGGTTC

ISSN: 1297-9716