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Table 3 Antimicrobial activities of the equine a-defensin DEFA1

From: Antimicrobial peptides and proteins of the horse - insights into a well-armed organism

Test strain

LD90 [μg/mL]

MBC [μg/mL]

Gram-negative bacteria

  

   E. coli ATCC 11775

0.8

1.6

   E. coli ATCC 25922

0.8

1.6

   E. coli ATCC 35218

0.2

1.6

   E. coli D31

1.6

3.1

   Kl. pneumoniae ATCC 13883

12.5

> 25*

   Enterobacter cloacae ATCC 13047

25

> 25*

   Ps. aeruginosa ATCC 10145

3.1

> 25*

   Ps. aeruginosa NCTC 11440

6.2

12.5

   Burkholderia cepacia ATCC 25416

> 25*

> 25*

   Salmonella choleraesuis subsp. typhimurium serovar Typhimurium

5

10

   P. multocida subsp. multocida

> 10*

> 10*

Gram-positive bacteria

  

   Staph. aureus ATCC 6538

1.6

3.1

   Staph. aureus ATCC 12600

1.6

6.2

   Staph. epidermidis ATCC 14990

3.1

6.2

   Enterococcus faecalis ATCC 29212

0.8

25

   Enterococcus faecalis PEG 205 (wild type)

1.6

12.5

   Strept. pyogenes ATCC 12344

3.1

3.1

   Strept. equi subsp. zooepidemicus

2.5

5

   Strept. equi subsp. equi

2.5

5

   Strept. dysgalactiae subsp. equisimilis

5

10

   Rh. equi ATCC 33701 P-

2.5

5

   Rh. equi ATCC 33701 P+

2.5

5

   Rh. equi 85 FP+

2.5

5

   Bacillus megaterium ATCC 14581

0.2

0.8

Yeast

  

   C. albicans ATCC 24433

3.2

3.2

  1. The antimicrobial activities of DEFA1 were determined by micro dilution assays against different bacterial strains and C. albicans. LD90 values (90% lethal dose) and MBC values (minimal bactericidal concentration; ≥ 99.9% killing) are given. * = The maximal peptide concentration used in the assay was still below the LD90 or MBC, respectively.
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Veterinary Research

ISSN: 1297-9716