Comparison of mature a-defensin amino-acid sequences of the horse. Sequences of a-defensin precursors that share 100% identity within the mature peptide were combined, e.g., DEFA1 and DEFA2 = DEFA1/2. Gaps introduced to maximize amino-acid alignments are indicated by hyphens. Conserved residues are highlighted: cysteine residues are colored red; arginine and glutamic acid, forming an intramolecular salt bridge, are colored light grey; glycine is marked in dark grey. Defensin-like peptides are shown in italics. Premature stop codons are indicated by asterisks. Numbers at the end of each sequence indicate the total number of amino acids followed by the net charge at pH 7.5. DEFA37L is not shown because of a premature stop codon in the propeptide.