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Figure 3 | Veterinary Research

Figure 3

From: Malignant catarrhal fever induced by Alcelaphine herpesvirus 1 is characterized by an expansion of activated CD3+CD8+CD4- T cells expressing a cytotoxic phenotype in both lymphoid and non-lymphoid tissues

Figure 3

CD8+CD4- T cells are the prominent cellular phenotype in lymphoid and non-lymphoid organs during WD-MCF in rabbits. At time of euthanasia, analysis by multi-colour flow cytometry was conducted on PBMC and mononuclear leukocytes isolated from pLN, spleen, liver, lung and kidney of mock-infected or AlHV-1-infected rabbits developing WD-MCF. (A) Flow cytometry dot plots obtained by multi-colour staining of PBMC and mononuclear leukocytes isolated from pLN, spleen, liver, lung and kidney of one representative rabbit of each group at time of euthanasia. (B) Relative percentages of B cells (IgM+), monocytes (CD14+), T cells (T-lymphocytes+), CD4+ T cells and CD8+ T cells in the lymphocyte gate are shown on the left panel. Relative percentages of CD4+CD8-, CD4-CD8+, CD4+CD8+, CD4-CD8- T cells are shown on the right panel. The percentages of T-lymphocytes-positive cells in the kidney of mock-infected animals were below 3% and were therefore not plotted. Data are representative of two independent experiments (n = 3). Bars show means ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.

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Veterinary Research

ISSN: 1297-9716