Novel gene expression responses in the ovine abomasal mucosa to infection with the gastric nematode Teladorsagia circumcincta

Table 2 Hybridisation design

Experiment	Hybridisations (d = days post-challenge)
Expt. 1	Nvd0/Nvd5	Nvd5/Imd5
Day 5 post-challenge	(n = 6)	(n = 6)
Expt. 2	Nvd2/Nvd0	Imd0/Nvd0	Imd2/Imd0
Day 2 post-challenge	(n = 5^a)	(n = 5^a)	(n = 5^a)

Samples in both cDNA hybridisation experiments were paired as indicated with a dye-balanced arrangement (three cy3/cy5 & three cy5/cy3 hybridisations). Nv = "naïve" yearlings, worm naïve prior to challenge; Im = "immune" yearlings, previously infected by trickle infection prior to challenge (see Table 1). Common samples linked the sets of paired hybridisations (Expt 1: Nvd5 samples, Expt. 2: Nvd0 & Imd0 samples). An indirect comparison between the results from all three hybridisations gave the (Nvd2/Nvd0)/(Imd2/Imd0) gene list. ^aOne chip failed hybridisation QC in Expt 2 and so was removed from the analysis, along with chips linked to it.

ISSN: 1297-9716