Extensive production of Neospora caninum tissue cysts in a carnivorous marsupial succumbing to experimental neosporosis

Table 1 Summary of experimental Neospora caninum infection in the fat-tailed dunnart.

Experimental dose, route	Id	Euthanasia	PCR	cELISA (%I)	IHC
10⁴ N. caninum tachyzoites, i.p.	A1	28 dpi	Neg.^b	Pos. (74%)	Neg.
	A2	28 dpi	Neg.^b	Pos. (41%)	Neg.
	A3	28 dpi	Neg.^b	Neg. (8%)	Neg.
10⁵ N. caninum tachyzoites, i.p.	B₁1	16 dpi^a	Pos.^c	Pos. (43%)	Pos.
	B₁2	18 dpi^a	Pos.^c	Pos. (42%)^f	Pos.
	B₁3	18 dpi^a	Pos.^c	Pos. (51%)^f	Pos.
	B₂1	13 dpi^a	Pos.^c	Neg. (25%)	Pos.
	B₂2	14 dpi^a	Pos.^c	Pos. (51%)	Pos.
	B₂3	13 dpi^a	Pos.^c	Neg. (19%)	Pos.
20-40 N. caninum oocysts, p.o.	E1	46 dpi	Neg.^d	Neg. (-4%)	Pos.^g
	E2	46 dpi	Pos.^e	Neg. (4%)^f	Neg.

^aanimal euthanised due to clinical neosporosis;
^bDNA isolated from brain, liver and lung tested;
^cDNA isolated from brain tested;
^dDNA isolated from brain, liver, spleen and lung tested;
^eDNA isolated from brain, liver, spleen and lung tested, positive PCR on brain;
^fsera diluted 1:5 due to limited amount of sera;
^gIHC positive N. caninum stages in spleen (all other organs tested N. caninum IHC negative).

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