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Table 1 Amplification of PrPSc from sheep faeces

From: Detection of prions in the faeces of sheep naturally infected with classical scrapie

Origin of faecal sample Animal ID number positive/total number of analyses Method 1a number of positive/total number of analyses Method 2b
  R382 0/6 0/2
  R328 0/6 0/2
  R234 0/6 0/2
  R413 0/6 0/2
  R421 0/6 0/2
Unexposed sheep R185 0/6 0/1
  R223 0/6 0/1
  R233 0/6 0/1
  R297 0/6 0/1
  R303 0/6 0/1
  Total 0/60 0/15 c
  1203/09 0/2 2/2 (4,4)
  1236/09 1/2 (9)d 1/2 (4)
  1252/09 0/2 2/2 (4,4)
  1271/09 0/2 2/2 (4,4)
  1284/09 0/2 2/2 (4,4)
  1292/09 0/2 2/2 (4,4)
Preclinical exposed sheep age 9-10 months 1298/09 0/2 1/2 (4)
  1305/09 2/2 (7,10) 1/2 (4)
  1227/09 0/2 1/1 (4)
  1279/09 0/2 1/1 (4)
  1286/09 0/2 1/1 (4)
  1296/09 0/2 1/1 (4)
  1299/09 0/2 1/1 (4)
  1307/09 0/2 1/1 (4)
  Total 3/28 19/22
  317/09 0/2 0/2
  319/09 0/2 1/2 (4)
  320/09 2/2 (6,6) 1/2 (4)
  324/09 1/2 (6) 2/2 (3,4)
  421/09 0/2 0/2
  352/09 0/2 0/2
  353/09 0/2 0/2
Sheep with clinical scrapie 354/09 0/2 0/2
  359/09 0/2 1/2 (4)
  382/09 0/2 0/2
  457/09 0/2 0/2
  470/09 2/2 (8,9) 2/2 (4,4)
  606/09 0/2 0/1
  608/09 0/2 1/1 (4)
  572/09 2/2 (7,9) 0/1
  Total 7/30 8/27
  1. aMethod 1: Prion was extracted in NP40 and DOC, precipitated with SiO2 and amplified by sPMCA for 10 rounds.
  2. bMethod 2: Prions were extracted in SDS, NaPTa precipitated and amplified by sPMCA in the presence of polyA for 4 rounds.
  3. cFaeces from a further 15 animals was analysed in duplicate by this method and no prions were detected (data not shown).
  4. dThe sPMCA round at which the sample first became positive is indicated in parenthesis.