Restriction fragment analysis of full-length BAC clone pBRf. A. BAC DNA was isolated and digested with seven different enzymes (HindIII, KpnI, PstI, SacI, SphI, StuI, XhoI) and separated on a 0.8% agarose gel. Sizes of a molecular weight marker (generuler 1-kb plus DNA ladder, Fermentas) are given. B. Patterns corresponded exactly to the predictions based on the complete CPXV.BR genome (#NC_003663) as shown by in silico digests using VectorNTI.