Table 5 Dual-luciferase assay groups (miRNA-Bcl2 mRNA target pairs).
Groups | Components |
|---|---|
Group 1 | Control (untransfected cells) |
Group 2 | pmirGLO + miR-153 mimics |
Group 3 | pmirGLO + miR-153 mimics NC |
Group 4 | pmirGLO + miR-153 inhibitor |
Group 5 | pmirGLO + miR-153 inhibitor NC |
Group 6 | pmirGLO-Bcl2 3′UTR-WT + miR-153 mimics |
Group 7 | pmirGLO-Bcl2 3′UTR-WT + miR-153 mimics NC |
Group 8 | pmirGLO-Bcl2 3′UTR-WT + miR-153 inhibitor |
Group 9 | pmirGLO-Bcl2 3′UTR-WT + miR-153 inhibitor NC |
Group 10 | pmirGLO-Bcl2 3′UTR-Mut + miR-153 mimics |
Group 11 | pmirGLO-Bcl2 3′UTR-Mut + miR-153 mimics NC |
Group 12 | pmirGLO-Bcl2 3′UTR-Mut + miR-153 inhibitor |
Group 13 | pmirGLO-Bcl2 3′UTR-Mut + miR-153 inhibitor NC |