Table 2 Primers of the detected genes for qPCR.
Gene name | Primers | Primer sequence (5′–3′) | Accession Number |
|---|---|---|---|
Bcl-xL | Forward | 5′-GTGCGTGGAGAGCGTAGACAAG-3′ | NM_214285.1 |
Reverse | 5′-ACCATCGGTTGAAGCGTTCCTG-3′ | ||
Bax | Forward | 5′-ATCTACCAAGAAGTTGAGCGAGTGT-3′ | XM_003127290.5 |
Reverse | 5′-CCAGTTGAAGTTGCCGTCAGC-3′ | ||
BAD | Forward | 5′-ACCGAGGAGGATGAAGGGACTGA-3′ | XM_021082883.1 |
Reverse | 5′-GGAACCCTGGAACTCGTCACTCA-3′ | ||
BID | Forward | 5′-CCGCACAGTTCAGGAACCAGAG-3′ | NM_001030535.1 |
Reverse | 5′-GCCAGGAGCATAGTCAGCACAAG-3′ | ||
Caspase 9 | Forward | 5′-CCTTCCTGTGTTCATCTCCTGCTTA-3′ | XM_003127618.4 |
Reverse | 5′-TGGCTCCTCTGGCTTGAGTTCC-3′ | ||
Caspase 3 | Forward | 5′-GCCGAGGCACAGAATTGGACT-3′ | NM_214131.1 |
Reverse | 5′-TTCGCCAGGAATAGTAACCAGGTG-3′ | ||
ERK1 | Forward | 5′-ACGACCACATCTGCTACTTCCTCT-3′ | XM_021088019.1 |
Reverse | 5′-CCACATATTCCGTCAAGAAGCCAGT-3′ | ||
ERK2 | Forward | 5′-CAGCACCTCAGCAACGACCATATC-3′ | NM_001198922.1 |
Reverse | 5′-GTGTTGAGCAGCAGGTTGGAAGG-3′ | ||
MEK1 | Forward | 5′-TGGAGGTGTGGTGTTCAAGGTCT-3′ | NM_001143716.1 |
Reverse | 5′-ATCTCGCCATCGCTGTAGAATGC-3′ | ||
p38 | Forward | 5′-ACGAGACCTCCGCCTGTGAA-3′ | NM_001243673.1 |
Reverse | 5′-TCCTCTTCCTGTCCTCCACCTTC-3′ | ||
p53 | Forward | 5′-ACCGCCGCACAGAGGAAGAA-3′ | NM_213824.3 |
Reverse | 5′-CGTCATTCAGCTCTCGGAACATCTC-3′ | ||
PI3K | Forward | 5′-ACAGCAAGCAGCACGAGTAACC-3′ | NM_001244503.1 |
Reverse | 5′-GCCTGGACTCCGACTTCTGGTAA-3′ | ||
AKT | Forward | 5′-CGGCAGGAGGAAGAGATGATGGA-3′ | NM_001159776.1 |
Reverse | 5′-AGCAGCTTCAGGTACTCGAACTCA-3′ | ||
GAPDH | Forward | 5′-GGTGAAGGTCGGAGTGAACG-3′ | NM_001206359.1 |
Reverse | 5′-CCGTGGGTGGAATCATACTGG-3′ |