Figure 6

Effects of miR-153 on apoptosis-regulated signalling pathways in IPEC-J2 cells. IPEC-J2 cells were transfected with miR-153 mimics or transfected with miR-153 inhibitor and then incubated with TsExos. Then, the assays were carried out. The protein expression levels of key effectors in the MAPK, p53, and PI3K/AKT signalling pathways were evaluated using Western blotting (A, C, E) and qPCR (B, D, F). All assays were performed in triplicate, and data are presented as the mean ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001 indicate the significance levels of the comparisons between the mimic NC group and the mimic group, as well as between the inhibitor NC group and the inhibitor group.