Infection of equine monocyte-derived macrophages with an attenuated equine infectious anemia virus (EIAV) strain induces a strong resistance to the infection by a virulent EIAV strain
© Ma et al. 2014
Received: 7 November 2013
Accepted: 23 July 2014
Published: 9 August 2014
The Chinese attenuated equine infectious anemia virus (EIAV) vaccine has successfully protected millions of equine animals from EIA disease in China. Given that the induction of immune protection results from the interactions between viruses and hosts, a better understanding of the characteristics of vaccine strain infection and host responses would be useful for elucidating the mechanism of the induction of immune protection by the Chinese attenuated EIAV strain. In this study, we demonstrate in equine monocyte-derived macrophages (eMDM) that EIAVFDDV13, a Chinese attenuated EIAV strain, induced a strong resistance to subsequent infection by a pathogenic strain, EIAVUK3. Further experiments indicate that the expression of the soluble EIAV receptor sELR1, Toll-like receptor 3 (TLR3) and interferon β (IFNβ) was up-regulated in eMDM infected with EIAVFDDV13 compared with eMDM infected with EIAVUK3. Stimulating eMDM with poly I:C resulted in similar resistance to EIAV infection as induced by EIAVFDDV13 and was correlated with enhanced TLR3, sELR1 and IFNβ expression. The knock down of TLR3 mRNA significantly impaired poly I:C-stimulated resistance to EIAV, greatly reducing the expression of sELR1 and IFNβ and lowered the level of infection resistance induced by EIAVFDDV13. These results indicate that the induction of restraining infection by EIAVFDDV13 in macrophages is partially mediated through the up-regulated expression of the soluble viral receptor and IFNβ, and that the TLR3 pathway activation plays an important role in the development of an EIAV-resistant intracellular environment.
Equine infectious anemia virus (EIAV) is an equine lentivirus with a tropism primary for monocyte/macrophage lineage in vivo ,. The clinical manifestation of equine infectious anemia (EIA), which is caused by EIAV infection, can be divided into an acute phase, a chronic phase, and an asymptomatic phase. The acute and chronic phases exhibit typical viremia accompanied by a high fever, anemia, thrombocytopenia, edema, and weight loss. The infected equines usually enter the life-long asymptomatic carrier state after 8–12 months. However, the virus maintains a low level of stable replication in tissues that are enriched with monocytes ,,. Stress or immunosuppression can increase the EIAV replication level in asymptomatic carriers and thus lead to a recurrence of EIA. Because most EIAV-infected equines become asymptomatic carriers due to immune control of the infection, EIAV has become a unique lentivirus model for studies investigating the immune control of lentivirus infection and its pathogenesis. In addition, most asymptomatic EIAV-infected horses demonstrate a significant resistance to infections caused by different pathogenic EIAV strains ,. This finding suggests that the EIAV system can provide a model for studying key immune factors that are involved in resistance to lentiviral infection, which can be used for the research and development of preventive lentivirus vaccines.
EIAVFDDV13 is an attenuated EIAV vaccine strain that induces immune protection in approximately 80% of vaccinated animals in laboratory and clinical studies ,. An understanding of the mechanism that underlies the induction of immune protection imparted by this attenuated vaccine strain will be useful for elucidating the immune protective mechanism that is responsible for lentivirus infection. Furthermore, the induction of immune protection results from the interaction between viruses and hosts. Therefore, the cellular responses of equine macrophages, the primary target cells of EIAV in vivo, should be evaluated after being infected by EIAV.
In this study, we examined the infection characteristics of a pathogenic EIAV strain EIAVUK3 on equine macrophages pre-infected by EIAVFDDV3 in vitro. We confirmed that EIAVFDDV13 induced a strong resistance to the subsequent EIAVUK3 infection in equine macrophages. Noticeably, in addition to the previously reported mechanism, i.e. masking viral receptor ELR1 by the SU protein of EIAV ,, our results revealed that up-regulation of the soluble EIAV receptor and interferonβ (IFNβ) by activated Toll-like receptor 3 (TLR3) are also largely involved in the resistance to EIAVUK3 infection induced by EIAVFDDV13.
Materials and methods
Cells and viral strains
eMDM and fetal donkey dermal (FDD) cells were used in this study as target cells for EIAV. The eMDM were prepared from the PBMC of one donor horse. The red blood cells (RBC) of equine animals have a faster sedimentation velocity than the white blood cells (WBC) in natural sedimentation in heparinized whole blood without any other treatment. After at least 30 min natural sedimentation, RBC will stay in the bottom of the flask, but most WBC including monocytes will stay on the upper layer with the plasma. Thus, the upper plasma layer supernatant including WBC was obtained from freshly collected, heparinized whole horse blood following natural sedimentation at room temperature for 30 min. The blood cells in the supernatant were isolated with centrifugation at 1000 rpm. After 2–3 washes with cold phosphate-buffered saline (PBS), the cells were incubated in RPMI 1640 culture medium (Gibco: Invitrogen Corporation, Carlsbad, CA, USA) supplemented with 10% horse serum (Hyclone, Logan, USA) and 40% fetal bovine serum (FBS) (Hyclone) at 37 °C and 5% CO2. After 24 h of incubation, non-adherent and loosely adherent cells were removed by washing with cold PBS quickly for three times. The remaining adherent cells were detached with normal saline at 37 °C and seeded into 96-, 24-, or 6-well microplates (Costar, Corning, USA) for 24 incubation at 1 × 105, 1 × 106, and 5 × 106 cells/well, respectively, depending on the experiment. After 48 h incubation, most adherent cells had differentiated into macrophages (see Additional file 1 to identify the differentiation from monocytes to macrophages by specific immune-staining) and were further used for EIAV infection assays. The FDD cell cultures were prepared and stored in our laboratory. FDD cells were prepared from EIAV negative fetal donkeys and cultured in minimal essential medium (α-MEM, Gibco) containing 2 mM L-glutamine, 10% heat-inactivated FBS, 100 IU penicillin, and 100 μg/mL streptomycin (Gibco) as previously described .
Two EIAV strains were used in this study. EIAVFDDV13, an attenuated vaccine strain of EIAV, was developed by passaging EIAVDLV121, a Chinese donkey leukocyte-adapted attenuated strain of EIAV, in FDD cells for 13 generations. A protective test demonstrated that EIAVFDDV13 induced protection from disease in approximately 80% of vaccinated horses , and the strain remained stably attenuated in the hosts . EIAVUK3, a pathogenic strain of EIAV, is an infectious clone constructed from the backbone of an EIAVwyoming strain . This infectious clone was kindly provided by Dr R. Montelaro of the Center for Vaccine Research at the University of Pittsburgh. The genomic variation at the nucleotide level between EIAVFDDV13 and EIAVUK3 was approximately 25%.
Quantification of EIAV load and detection of viral replication
Real-time quantitative reverse transcription PCR (RT-PCR) and reverse transcriptase activity (RT) assays were used to identify the EIAV load. The relative titers of EIAVFDDV13 and EIAVUK3 were comparable and consistent when measured with these methods. Therefore, a quantitative RT-PCR assay of viral genomic RNA was used to quantify the loads of EIAVFDDV13 and EIAVUK3 according to previously described procedures ,.
The infectious titer of the two EIAV strains was tested using the median tissue culture infective dose method (TCID50). Fifty microliters of viral supernatants that were serially diluted by 10−4, 10−5, 10−6, and 10−7 was added to eight wells of 96-well flat-bottom plates; each well contained 5 × 105 FDD cells. After an initial incubation of 2 h, viruses in the culture medium were removed with three washes with serum-free medium. Fresh cell culture medium was then added to the cultures, and the infected cells were incubated continuously. Four days later, EIAV growth was monitored by measuring viral RT activity using a Roche RT detection kit (Roche, Basel, Switzerland). Optical density values two-fold higher than those determined for the negative control were considered to indicate viral replication. The TCID50 value of the virus was determined as described by Reed and Muench .
To examine the proliferation profiles of EIAV in eMDM, 1 × 105 cells were infected with 1 × 103 TCID50 of EIAVFDDV13 (amounted to approximately 1 × 107 viral RNA copies of EIAVFDDV13) or 1 × 103 TCID50 of EIAVUK3 in a 96-well microplate as indicated in the text. The culture medium was exchanged for fresh culture medium after 2 h of infection. The cells in some wells were used to determine the intracellular viral RNA copies at 3 h after infection by EIAV. These cells were washed three times with PBS and treated with trypsin-EDTA (0.25% trypsin, 5 mM EDTA) at room temperature for 5 min to remove adherent virus that had not entered the cells. The other cells that were used to examine the proliferation profiles were further incubated for 2, 3, 4, 5, 6, or 7 days, after which the cell culture supernatants were collected. Triplicate wells were used for each detection time point. Total RNA was extracted from the harvested cells and culture supernatants using Trizol (Invitrogen, Carlsbad, CA, USA) or the QIAamp Viral RNA Mini Kit (Qiagen, Hilden, Germany) and was processed for cDNA synthesis using M-MLV reverse transcript kit (Invitrogen) using 100 ng of RNA template. The cDNA obtained was used for qPCR analysis. The replication kinetics of the viruses was determined in three independent experiments.
Co-infection measured by RNA HIS
EIAV positive-strand RNA in infected cells was detected with a QuantiGene ViewRNA Plate-based Assay Kit (Panomics, Silicon Valley, USA). Two sets of specific probes that targeted the EIAV genome at nucleotides 2065 to 3210 of EIAVFDDV13 (GenBank accession # GS00329) and nucleotides 1,210 to 2356 of EIAVUK3 (GenBank accession # AF016316) were designed and provided by Panomics. The divergences between the two targeted regions of these two EIAV strains (EIAVFDDV13 and EIAVUK3) are 23.1% and 27.6%, respectively. The eMDM were plated in 96-well plates (Costar) and simultaneously infected with these two EIAV strains. At 48 h after initial EIAV infection, the cells were fixed with 4% formaldehyde and dehydrated in ethanol. During the detection process, the cells were rehydrated, permeabilized, digested with protease and hybridized with the specific probes as recommended by the manufacturer. Confocal microscopy and image acquisition were performed with a Leica TCS SP5 (Leica, Wetzlar, Germany).
Measurement of mRNA expression by the branched DNA technique and real-time quantitative RT-PCR
The branched DNA (bDNA) technique was used to measure the expression levels of multiple genes in the cultured cells. The specific oligonucleotide probe sets for the target genes included equine TLR3, TLR7, TLR8, TLR9, IFNα1, IFNβ, ELR1, and β-actin, which were used with the QuantiGene 2.0 Reagent Systems designed and provided by the manufacturer (Panomics). Information regarding the probe sets is provided in Additional file 2. The amounts of multiple target mRNA in each sample were simultaneously determined by measuring the wavelengths of color-coded microspheres and the intensities of the luminescent emission of streptavidin-conjugated R-phycoerythrin using a Luminex 200 (Molecular Devices, Silicon Valley, USA). All data obtained from the Luminex 200 were analyzed using the Luminex IS2.3 program. A total of 100 events per region were collected. For all of the samples analyzed with the bDNA assay, background signals determined in the absence of target mRNA were subtracted from the signals obtained in the presence of target mRNA. The expression levels of the intracellular mRNA were normalized to β-actin. Changes in gene expression were calculated by the following method: fold changing value = (copies of target mRNA/copies of β-actin mRNA)treated sample/(copies of target mRNA/copies of β-actin mRNA)untreated control, and were presented as the log2 mean fold changing value in the results. The ratio of copies between target mRNA and β-actin mRNA for the untreated control was used as the calibrator and assigned a fold-change expression value of 1. Three independent experiments were performed for each treatment. In addition, the gene expression of the “house-keeping” β-actin gene used in this study was detected and compared among different eMDM: those infected with EIAVFDDV13 and EIAVUK3 or treated with Poly I:C. The eMDM under different treatments were harvested at different internal times and counted. eMDM with the same numbers were used to quantify mRNA copies of β-actin by Quantitative real-time RT-PCR.
Quantitative real-time RT-PCR using a kit Platinum® SYBR® Green qPCR SuperMix-UDG (Invitrogen) which utilizes SYBR Green as a detector was performed by using an MxPro 3005p qPCR system (Stratagene, La Jolla, CA, USA) to analyze the expression of ELR-IN, an alternative splicing isoform of the EIAV receptor ELR1 , TLR3, and β-actin. Primers were designed based on the nucleotide sequence of ELR-IN (GenBank accession # EF190264) to specifically distinguish ELR-IN from ELR1. The sequences of the primers were IN-FW, 5′ GGAGAGTCCTTCAGACCTGAGTTCAC3′; IN-RV, 5′ CGCTGCACCTAGGAGAGAAGATTGGC3′. The primers for TLR3 mRNA (GenBank accession # NM_001081798.1) were TLR3-FW, 5′ GGGCAAGAACTCACAGGTCAG 3′; TLR3-RV, 5′ CAAACCAGGCAATGCTTTCAC 3′. The primers for β-actin mRNA were F2, 5′ CGACATCCGTAAGGACCTGTA 3′; R2, 5′ CATCTGCTGGAAGGTGGACAA 3′. Total RNA was extracted from the harvested cells using Trizol (Invitrogen) and was processed for cDNA synthesis using an M-MLV reverse transcript kit (Invitrogen,) using 100 ng of RNA template. The cDNA obtained was used for qPCR analysis. qPCR was conducted under the following conditions suggested by the manufacturer of Platinum SYBR Green qPCR SuperMix-UDG kit (Invitrogen): initial preincubation at 50 °C for 20 s; 95 °C for 10 min; 40 cycles of 95 °C for 30 s and 60 °C for 1 min; and one cycle of 95 °C for 1 min, 55 °C for 30 s and 95 °C for 30 s for signal sampling. Linear regression analysis of the standard curve and the β-actin values was used to estimate the ELR-IN and TLR3 mRNA level in the samples.
Enzyme-linked immunosorbent assay for equine IFN-α/β
Enzyme-linked immunosorbent assays (ELISA) for the analysis of equine IFNα and IFNβ proteins were performed as described in the protocol provided by the manufacturer (Uscn Life Science, Wuhan, China). The plate was read with a microplate reader VERSAmax (Molecular Devices). The protein expression levels of IFN were measured by ELISA as pg/mL calibrated with a set of standards provided by this kit, and the changes in protein expression levels in each sample were calculated using the following formula: fold changing value = the amount of target protein in the treated samples/the amount of target protein in untreated samples, and the mean values of fold change were converted to log2. The amount of IFN proteins in the untreated control was used as the calibrator and assigned a fold-change expression value of 1. All reactions were performed in triplicate.
EIAV infection of poly I:C-treated eMDM
eMDM were cultivated in 96-well plates and treated with either 0.5 μg/mL poly I:C (Sigma-Aldrich, St Louis, USA) or the same volume of PBS as a control. The culture supernatant was removed 12 h after the treatment, and fresh medium was added. The cells were then infected with 1 × 103 TCID50 of EIAVUK3. After 2 h of incubation, the culture medium was removed, and the cells were washed three times with PBS before incubation in fresh medium for an additional 72 h. The viral copy numbers in the culture medium of triplicate wells were quantitatively analyzed by qPCR.
RNA interference of TLR3 expression
To knock down TLR3 expression, a TLR3-specific small interfering RNA (siRNA-TLR3) and a control siRNA (siRNA-C) with scrambled sequences were synthesized by RiboBio, Guangzhou, China. The target sequence was 5′-GGACCTTGGCCTTAATGAA-3′, and the product number for siRNA-C was Ncontrol_05815. Primary eMDM were plated in 96-well plates at 1 × 105/well and transfected with either 50 nM siRNA-TLR3 or 50 nM siRNA-C using the transfection reagent FuGENE HD (Promega, Madison, USA) according to the manufacturer’s protocol. The cells were harvested at 48 h after transfection and evaluated for the efficacy of TLR3 mRNA knockdown.
All experiments in the present study were replicated at least three times unless specifically indicated. The results in the figures are presented as the mean ± SEM. Significant differences between samples or groups were determined with the student’s t-test. The statistical analysis was performed using SAS 8.1 software.
EIAVFDDV13 induced strong resistance to the infection of EIAVUK3 on eMDM
Afterwards, the restriction of a subsequent infection with EIAVUK3 by pre-infection with EIAVFDDV13 or a subsequent infection with EIAVFDDV13 by pre-infection with EIAVUK3 was investigated. The viral RNA copy numbers of EIAVUK3 or EIAVFDDV13 in eMDM pre-infected with EIAVFDDV13 for 6, 12, 24, 36 and 48 h were measured and compared with the EIAV RNA copy numbers in control groups (cells not treated with pre-infection of EIAV). As shown in Figure 1D, the RNA copy numbers of EIAVUK3 were markedly reduced by 92.68 ± 3.35% by prior infection with EIAVFDDV13 for 6 h compared with the control group. The restriction effect increased by approximately 5-fold with prolonged pre-infection time until 24 h, at which point the reduction in RNA copy number was 98.71 ± 0.48%. In contrast, the resistance induced by EIAVUK3 to subsequent infection by EIAVFDDV13 was much weaker. The restriction induced by EIAVUK3 was approximately 85% throughout the detection period and was 10-20-fold lower than that induced by EIAVFDDV13 after 24 h of pre-infection. Meanwhile, there was no significant difference in apoptosis of eMDM induced by the infection of these two EIAV strains (see Additional file 3), which ruled out the possible effect of cell degradation on the aforementioned difference in viral RNA replication. These results demonstrate that the initial infection of EIAVFDDV13 in eMDM induced a strong resistance to the subsequent infection of EIAVUK3.
Infection of eMDM with EIAVFDDV13 and EIAVUK3 differentially influenced the expression of ELR1 and soluble ELR1 (sELR1)
We recently identified an alternative splicing variant of ELR1 transcripts that retained a fragment of intron 6. This sliced transcript, termed as ELR-IN (GenBank accession # EF190264), accounts for a large proportion of ELR1 transcript variants (approximately 50% of the ELR1 transcript) and creates an isoform with four different amino acid residues and then a premature stop codon 14 residues upstream of the predicted membrane spanning domain. The truncated ELR1 protein is predicted and confirmed as a soluble form of ELR1. A separate study revealed that sELR1 appeared to inhibit EIAV infection in cultivated host cells . Therefore, the regulation of ELR-IN expression by EIAV infection was tested. The levels of sELR1 mRNA in eMDM infected with EIAVFDDV13 were significantly higher than in EIAVUK3-infected cells and uninfected control cells within 12–24 h after infection, differently than that was observed for transmembrane ELR1 (Figure 2B). This result suggests that the up-regulation of sELR1 expression in eMDM after infection with EIAVFDDV13 may contribute to induction of infection resistance by this EIAV strain.
The expression levels of viral nucleic acid-recognizing TLR and type I interferons were differentially regulated by EIAVFDDV13 and EIAVUK3 infection in eMDM
Studies have shown that type I interferons are associated with intracellular TLR activation and that they are part of the downstream products of TLR signaling pathways –. Given the obvious up-regulation of TLR3 mRNA expression by EIAVFDDV13 infection and the antiviral activity of type I interferons, changes in the expression of IFNβ and IFNα in eMDM after infection with EIAVFDDV13 and EIAVUK3 were evaluated. As shown in Figure 3B, there were significant differences in IFNβ expression in eMDM infected with the two strains. At 24 h after the infection of eMDM with EIAVFDDV13, IFNβ expression was up-regulated by 10- to 20-fold at the mRNA level and 4- to 6-fold at the protein level compared with the mock-treated control; the timing of this up-regulation was correlated with the temporal up-regulation of TLR3 expression in eMDM infected with EIAVFDDV13. In contrast, IFNβ mRNA and protein expression in eMDM infected with EIAVUK3 did not differ significantly from those in the mock-treated control. Although EIAVUK3 infection up-regulated IFNα expression (approximate 3 folds in protein level), infection with EIAVFDDV13 basically did not (Additional file 6). Based on the antiviral effect of IFNβ, it is likely that the up-regulation of IFNβ expression is positively correlated with the strong infection resistance induced by EIAVFDDV13.
TLR3 activation in eMDM induced by poly I:C resulted in increased sELR1 and IFNβ mRNA expression
Intriguingly, following the specific activation of TLR3 by poly I:C in eMDM, the mRNA expression levels of both IFNβ and sELR1 were up-regulated. The up-regulation of IFNβ was similar to that observed in EIAVFDDV13-infected eMDM (Figure 4A). Moreover, expressions of IFNα and TLR7-9, which show lower intensity induced by attenuated EIAVFDDV13 than that induced by the virulent EIAVUK3 or show a limited difference between these two EIAV strains, were also not up-regulated after poly I:C treatment (data not shown). These results indicate that enhanced IFNβ and sELR1 expression can be induced by TLR3 activation, which in turn promotes resistance of the target cells to EIAV infection.
TLR3 activation in eMDM played an important role in induction of infection resistance to EIAVUK3
In this study, we found that in equine macrophages, EIAVFDDV13 infection could induce strong resistance to subsequent infection of the heterologous virulent EIAV strain EIAVUK3. Furthermore, we observed that some cellular factors involved in the activation of innate immunity by EIAVFDDV13, which occurred primarily through TLR3 activation, were important contributors to the development of infection resistance. Such host cell responses could disturb either the entrance or replication of the virus, which results in the decline of viral RNA copies in target cells. As observed in this study, the viral RNA copies of EIAVUK3 in the culture supernatant of macrophages preinfected with EIAVFDDV13 were noticeably lower than that in the un-preinfected controls.
The phenomenon that a virally infected cell becomes resistant to subsequent infection by the same or similar viruses is referred to as superinfection resistance (SIR) . In this study, Figure 1A shows that at least 10 copies of viral RNA were detected per cell 3 hpi. This level of detection suggests that every target cell was infected by EIAV prior to the second infection of EIAVUK3. Therefore, SIR is one of the possible mechanisms that takes part in the development of resistance to subsequent viral reinfection. The primary mechanism underlying SIR induction by HIV-1 is down-regulation of the expression of the principle HIV-1 receptor CD4 on the cell surface ,. In the present study, only limited up- or down-regulation on the mRNA level of EIAV receptor ELR1 was detected in eMDM infected with either EIAVFDDV13 or EIAVUK3. This observation is consistent with previous reports that the amount of membrane-bound ELR1 was not reduced by EIAV infection ,. In addition, these two EIAV strains acted similarly on ELR1 expression but induced TLR3 expression differently, which implicates that ELR1 does not play an essential role in the resistance to subsequent infection inducted by EIAVFDDV13. In contrast to intact membrane-bound ELR1, a 2- to 3-fold difference in the expression of sELR1, the soluble form of ELR1, was detected after infection of eMDM with EIAVFDDV13, but not EIAVUK3. Because soluble viral receptors generally exert inhibitory effects on viral infection – and sELR1 mRNA accounts for as much as 50% of the total ELR1 mRNA present (unpublished data), the changes in sELR1 expression observed after viral infection in this study are likely to be involved in the resistance induced by EIAV. Our results demonstrate that poly I:C stimulated sELR1 expression by specifically activating TLR3 and that sELR1 expression was not up-regulated by EIAVFDDV13 infection of eMDM after the knockdown of TLR3 mRNA. These data support the hypothesis that TLR3 pathway activation mediates the up-regulated expression of the soluble ELR1 receptor after EIAV infection. However, one should be cautious in evaluating the role of sELR1 in SIR induced by EIAVFDDV13 because of the observed modest up-regulation of sELR1 mRNA expression and the absence of confirmation at the protein level, which was precluded by the low native expression of sELR1 in eMDM and the lack of a specific antibody that differentiates sELR1 from the prototype ELR1. Beside this, the linkage between TLR3 activation and sELR1 expression is not clear.
In addition to the up-regulation of soluble viral receptors, the enhanced expression of innate immunity-related factors is another mechanism that restrains viral replication and protects cells from subsequent infection. In the present study, we focused on TLR3 and IFNβ. Our results show EIAVFDDV13 strongly stimulated TLR3, whereas EIAVUK3 did not. TLR3 activation in macrophages has been shown to prevent HIV-1 infection , suggesting that the activation of the TLR3 signaling pathway might help macrophages to resist subsequent infection with similar viruses. In this study, stimulating TLR3 expression by poly I:C effectively prevented EIAV infection of the target cells, and TLR3 knockdown with siRNA largely reduced the antiviral effect of poly I:C. Although other pattern recognition receptors (PRR), such as retinoic acid-inducible gene protein I (RIG-I) and melanoma differentiation-associated protein 5 (MDA-5), belong to the RIG-I-like receptor (RLR) family and might also be involved in the innate immune response against EIAV infection, our results indicate that enhancing TLR3 expression alone effectively improved the ability of macrophages to resist to EIAVUK3 infection. On the contrary, our results also show that the resistance to subsequent infected EIAVUK3 induced by a TLR3 ligand, poly I:C, was noticeably lower than that induced by EIAVFDDV13 (90% vs 98% of inhibition), and the knockdown of TLR3 expression by siRNA only reversed 10-20% resistance to the subsequently infected virus. Even though the incomplete interference of TLR3 expression (about 65%) partially counts for the incomplete reverse of the EIAVFDDV13-induced SIR, other mechanisms may also be involved in, such as interfering viral entrance by the binding of EIAV gp90 surface protein to the membrane-bound receptor ELR1 ,.
Following TLR3 activation by EIAVFDDV13, IFNβ production in eMDM also significantly increased, which was consistent with IFNβ as a major downstream product of this PRR ,. Considering the important role of IFNβ in innate anti-virus function, the significantly up-regulated IFNβ expression observed in eMDM infected with EIAVFDDV13 is considered a key contributor in the infection resistance induced by this virus. In addition to their role in antiviral function, TLR3 and IFNβ also play important roles in specific adaptive immune responses. In addition to their role in antiviral function, TLR3 and IFNβ also play important roles in specific adaptive immune responses. These include the promotion of T cell-dependent and -independent antibody responses in follicular B cells ,, the promotion of germinal center formation, the production of neutralizing antibodies  and the stimulation of the development of lymph node-resident T follicular helper cells . These activated immunocytes are critical for the germinal center reaction and humoral immunity response . Therefore, the enhanced up-regulation of TLR3 and IFNβ expression induced by EIAVFDDV13 likely contributes to the development of specific immunity against EIAV that is elicited in vivo through the inoculation with the attenuated strain.
Although the same amounts of initial viral titers were added, which were normalized by both TCID50 and viral RNA copy number, and these two viruses had similar replication kinetics and infectivity, EIAVFDDV13 and EIAVUK3 induced significantly different TLR3, sELR1 and IFNβ expression in eMDM. Our data indicate that besides the previous reported mechanism of competitive receptor binding by the viral surface protein, TLR3 pathway activation plays a vital role in the infection resistance induced by EIAVFDDV13 infection in macrophages in vitro. Therefore, these two viral strains should stimulate the dsRNA-recognizing TLR3 with different efficacies. Because the sequence variation in the genomes of EIAVFDDV13 and EIAVUK3 is approximately 25% ,, the dsRNA structures formed within the single-strained RNA viral genomes are considered different. In addition, EIAVFDDV13 consists of quasispecies with an average genomic diversity of approximately 3% while EIAVUK3 is derived from a proviral clone ,. These differences may influence the binding affinities of PAMP (dsRNA) for TLR3 in these two EIAV strains, and appear to account for the differences in PRR activation and the expression of TLR3-associated cytokines. Besides the aforementioned speculation, another aspect under consideration is that the virulent EIAVUK3 may have one or more mechanisms to block or dampen the early innate immune response. Exploration of EIAV-specific mechanisms that are responsible for the suppression of innate immunity by virulent strains should be highly informative. Consequently, the results of this study provide insights that will facilitate a better understanding of the interaction between host cells and EIAV, as well as other lentiviruses.
Our data demonstrate that sELR1 and IFNβ are up-regulated when TLR3 is activated and those cells in which TLR3 is activated show enhanced resistance to EIAVUK3 infection. Silencing TLR3 expression with siRNA significantly reduces this inhibitory effect. More importantly, infection resistance induced by EIAVFDDV13 is significantly reversed after TLR3 silencing. Based on the significant difference in TLR3 expression in eMDM stimulated with EIAVFDDV13 and EIAVUK3, we hypothesize that TLR3 pathway activation plays an important role in the induction of infection resistance by EIAVFDDV13 infection in macrophages in vitro.
Equine infectious anemia virus
Equine monocyte-derived macrophages
Toll like receptor
Median tissue culture infective dose method
Pattern recognition receptors
Pathogen associated molecular pattern
Fetal donkey dermal
Fetal bovine serum
Reverse transcriptase activity
Reverse transcription PCR
Enzyme-linked immunosorbent assays
We are especially grateful to Dr R. Montelaro of the Center for Vaccine Research at Pittsburgh University for providing the molecular clone of EIAVUK3. This study was supported by Grant 31372451 and 31222054 from the National Natural Science Foundation of China, Grant 2012ZX10001-008 from the Chinese National Key Programs for Infectious Diseases in China, Grant LBH-Q12001 from Postdoc Foundation of Heilongjiang province, China, and Grant SKLVBP2011 from State Key Laboratory of Veterinary Biotechnology, China.
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