Figure 1From: Inability of NS1 protein from an H5N1 influenza virus to activate PI3K/Akt signaling pathway correlates to the enhanced virus replication upon PI3K inhibitionEffects of exogenous NS1 proteins on Akt phosphorylation in different cells. Hela (A, B, C) or A549 cells (D) were serum-starved for 12 h and transfected with different NS1-expressing plasmids or PNF empty vector. At 24-h post transfection, cells were lysed and subjected to Western blot analysis using the specific antibodies for phospho-Akt(Ser473), total-Akt, β-actin, or NS1.Back to article page