Identification of a novel mechanism of action of bovine IgG antibodies specific for Staphylococcus aureus

Staphylococcus aureus is a major pathogen that causes subclinical mastitis associated with huge economic losses to the dairy industry. A few vaccines for bovine mastitis are available, and they are expected to induce the production of S. aureus-specific antibodies that prevent bacterial adherence to host cells or promote opsonization by phagocytes. However, the efficacy of such vaccines are still under debate; therefore, further research focusing on improving the current vaccines by seeking additional mechanisms of action is required to reduce economic losses due to mastitis in the dairy industry. Here, we generated S. aureus-specific bovine IgG antibodies (anti-S. aureus) that directly inhibited bacterial growth in vitro. Inhibition depended on specificity for anti-S. aureus, not the interaction between Protein A and the fragment crystallizable region of the IgG antibodies or bacterial agglutination. An in vitro culture study using S. aureus strain JE2 and its deletion mutant JE2ΔSrtA, which lacks the gene encoding sortase A, revealed that the effect of anti-S. aureus was sortase-A-independent. Sortase A is involved in the synthesis of cell-wall-associated proteins. Thus, other surface molecules, such as membrane proteins, cell surface polysaccharides, or both, may trigger the inhibition of bacterial growth by anti-S. aureus. Together, our findings contribute insights into developing new strategies to further improve the available mastitis vaccine by designing a novel antigen on the surface of S. aureus to induce inhibitory signals that prevent bacterial growth. Electronic supplementary material The online version of this article (10.1186/s13567-018-0517-y) contains supplementary material, which is available to authorized users.


Introduction
Mastitis is an inflammatory disease caused by infection in mammals, such as dairy cattle, by pathogenic microorganisms, such as Staphylococcus aureus [1]. Major economic losses incurred by the dairy industry are associated with mastitis [2]. Once S. aureus infects the mammary gland of dairy cattle, it can infiltrate deep into the tissue and survive elimination by phagocytic cells, such as macrophages [3]. Therefore, although antibiotics have been used to treat dairy cattle for mastitis caused by S. aureus, they do not effectively reach the lesion in most cases, making it difficult to achieve a complete cure [4,5]. S. aureus causes either subclinical or clinical mastitis characterized by abnormal milk containing a large number of somatic cells or by symptoms of per-acute or acute mastitis with pathogen-nonrelated common features, such as swelling, erythema, pain, and perception of heat, respectively [6].
Vaccination is an effective strategy to prevent inflammation, including mastitis. Vaccines, such as STARTVAC ® [7][8][9] and Lysigin ® [10], are used globally to protect dairy cattle from mastitis caused by S. aureus. These mastitis vaccines comprise killed S. aureus or S. aureus lysate and act primarily through the induction of antibodies: these may lead to antibody-mediated opsonization and removal of the pathogen through phagocytosis [7][8][9][10]. In fact, a recent study addressing the efficacy of STARTVAC ® revealed that it evokes the Th2 type of immune response that results in inducing S. aureusspecific IgG1 antibody production in not only serum but also milk [9]. Alternatively, it may be possible to inhibit attachment of S. aureus to mammary alveoli by binding of the vaccine-induced antibodies to one or more bacterial surface molecules that are involved in adhesion [7][8][9][10]. However, the economic loss of the last few decades due to mastitis has remained at approximately $2 billion per year in the US [11,12]. Therefore, further research is required to enhance the efficiency of mastitis vaccines.
Antibodies possess numerous immunological activities, and their roles are versatile because the animals vaccinated with whole killed bacteria possess polyclonal antibodies with high reactivity for multiple antigens expressed by the immunized bacteria [13]. Further, the feasibility of using antibodies that bind to the bacterial surface to inhibit growth remains to be evaluated. Therefore, inhibiting bacterial growth using antibodies has not been the focus of research compared with that aimed at inhibiting bacterial adhesion to their hosts [14,15]. However, a complete understanding of the anticipated effects of current vaccines may be important to decrease the morbidity and mortality caused by targeted diseases. Therefore, the purpose of the present study was to examine the role of S. aureus-specific antibodies in the inhibition of bacterial growth and define the underlying mechanisms.
Here we generated bovine polyclonal IgG antibodies specific for S. aureus (anti-S. aureus) by immunizing a Holstein calf with killed S. aureus. We demonstrate that the growth of S. aureus was inhibited in vitro by anti-S. aureus. These results may serve as a foundation for improving current mastitis vaccines to effectively control bacterial growth in vivo.

Bacteria
S. aureus (BM1006 [16], SA003 [17] and JE2 [18]), S. epidermidis (ATCC14990) [19], Bacillus atrophaeus (ATCC9372) [20], and E. coli (JM109) [21] were used in the present study. BM1006 was isolated from the bulk milk of dairy cattle, and SA003 originated from the milk of dairy cattle suffering from mastitis. S. aureus JE2 is extensively used in the laboratory. A transposon mutant derived from JE2 (NE1787, termed JE2ΔSrtA) [22] was used to investigate sortase-A-dependent cell-wall-associated proteins. The evolutionary relationships among the 16S ribosomal RNA genes of 21 bacteria and the Archaea Pyrococcus horikoshii (listed in Additional file 1) were inferred using the UPGMA method, and evolutionary distances were calculated using the Maximum Composite Likelihood method of MEGA7 as previously described [23,24].

Antibody production
A Holstein calf (5 months old, male) was subcutaneously immunized with formalin-killed S. aureus [BM1006, 1.5 × 10 10 colony-forming units (CFU)] together with TiterMax ® Gold (TiterMax) on three occasions at 2-week intervals. One week after the final immunization, serum was collected, and polyclonal IgG antibodies were purified using Protein G Sepharose 4 Fast Flow (GE healthcare). The concentration of IgG antibodies after purification was measured by BCA Protein Assay Kit (Thermo Fisher). The use of the Holstein calf to produce antibodies was performed in accordance with protocols approved by the Institutional Animal Care and Use Committee of Tohoku University.

Enzyme-linked immunosorbent assay (ELISA)
ELISA were performed to determine the amount and specificity of purified polyclonal bovine IgG antibodies. Briefly, 96-well plates (Nunc) were coated with 2 μg/mL of purified sheep anti-bovine IgG-heavy chain antibodies (Bethyl) overnight at 4 °C. Alternatively, the plates were coated with 5 μg protein/mL of either S. aureus, S. epidermidis, B. atrophaeus, or E. coli, each killed with 0.5% (w/v) formalin (Wako). After blocking with 0.05% (v/v) Tween-20 in Tris-buffered saline for 1 h at room temperature (RT), twofold serial dilutions of polyclonal IgG antibodies obtained from the immunized Holstein calf or control bovine IgG antibodies (Sigma-Aldrich) were incubated for 2 h at RT. After washing, HRP-conjugated sheep anti-bovine IgG-heavy chain antibodies diluted 1:10 000 (Bethyl) were treated for 1 h at RT, and the reactions were developed with a TMB microwell peroxidase substrate system (KPL).

Flow cytometry
Bacteria killed with 0.5% (w/v) of formalin were incubated for 30 min at 4 °C with 1000 μg/mL of either anti-S. aureus or control IgG, both of which were conjugated with FITC (Sigma-Aldrich), as described previously [25]. Untreated bacteria were prepared as a control. After washing, the bacteria were analyzed using a BD Accuri C6 Flow Cytometer (BD Bioscience), and the data were analyzed using FlowJo (Digital Biology).
The membrane was treated with 10 μg/mL of either the anti-S. aureus or control IgG antibody for 1 h at RT, after blocking the membrane with 0.05% (v/v) of Tween-20 in Tris-buffered saline overnight at 4 °C. After washing, the membrane was treated with HRP-conjugated sheep anti-bovine IgG-heavy chain antibodies diluted 1:10 000 (Bethyl) for 1 h at RT, and the reaction was developed using an EzWestLumi plus (ATTO).

Bacterial culture in vitro
Staphylococcus aureus (BM1006, SA003, JE2, and JE2ΔsrtA) and E. coli (JM109) were cultured overnight at 37 °C in Trypto-Soya (TS) broth (Nissui), and 40 μL was added to 4 mL of fresh TS broth containing anti-S. aureus (10, 100, and 1000 μg/mL) or control IgG (1000 μg/mL) to address the effect of anti-S. aureus on the bacterial growth. In some experiments, IgG antibodies in the used medium that originally included anti-S. aureus were re-used for the additional in vitro study to test the effect. Size and autofluorescence intensity of S. aureus cultured for 0.5, 2, 5, and 24 h in the presence of either anti-S. aureus or control IgG were analyzed using a microbial particle counter (Rion). In brief, the culture broth of S. aureus was diluted 1:100 000 with distilled water and subjected directly to microbial particle count analysis in which the scattered light and autofluorescence intensity mediated by Riboflavin derived from violet laser-exposed bacteria were measured. The morphology of S. aureus cultured for 0.5, 2, 5, and 24 h was analyzed using a scanning electron microscope (SEM). Specifically, the culture medium of S. aureus (BM1006) was removed after quick centrifugation for 1 min, and the remaining bacterial pellet was fixed with 2.5% (v/v) glutaraldehyde (Nacalai tesque) in 0.1 M phosphate buffer for 1 h at 4 °C. After washing the bacterial pellet to remove excess fixative, bacteria were suspended using distilled water and affixed to glass slides (Matsunami). The bacteria were then coated with platinum and palladium. Images of S. aureus were obtained using an SEM (SU8000, Hitachi) operated at 3.0 kV. Survival of bacterial cells at 0.5, 2, 5, and 24 h after the culture was determined by obtaining CFU. Specifically, aliquots of bacterial broth were collected and seeded on TS agar plates (three replicates) after dilution with saline. To determine the quantitative effect of anti-S. aureus in TS broth during bacterial culture, samples were collected at 0.5, 2, 5, and 24 h after the start of culture and filtered using 0.45-μm syringe filters (Advantec) to remove antibodies bound to bacteria. The pass-through solutions were subjected to ELISA analyses to determine both the total amount of IgG antibodies and the titer of S. aureusspecific antibodies after the use of antibodies as additive in the S. aureus culture. To address those, ELISA analyses were performed using 96-well plates coated with either purified sheep anti-bovine IgG-heavy chain antibodies (Bethyl) or killed S. aureus as described above.

Statistics
Statistical analyses were performed using one-way ANOVA with the Kruskal-Wallis test and two-way ANOVA with Tukey's multiple comparisons test using Prism 7 software (GraphPad).

Generation of bovine IgG antibodies against S. aureus
Since mastitis vaccines were not commercialized in Japan when we started this study, we first performed an immunization study with killed S. aureus prepared in our laboratory to reproduce the current mastitis vaccine trials that have been tested outside Japan. Specifically, a Holstein calf was subcutaneously immunized with killed S. aureus BM1006 that was isolated from bulk milk of dairy cattle and IgG antibodies were purified from the serum collected from the immunized calf. Antibody specificity was confirmed using an ELISA and the test antigens as follows: S. aureus, S. epidermidis, B. atrophaeus, and E. coli. Figure 1A shows a phylogenetic tree displaying the genetic similarity among bacteria used in this study and other well-characterized bacteria. The IgG antibodies reacted with BM1006 in a concentration-dependent manner; however, the control bovine IgG antibodies were not detectably reactive ( Figure 1B). We therefore defined the immunized and control IgG antibodies as anti-S. aureus and control IgG, respectively. Anti-S. aureus strongly reacted with S. aureus SA003 ( Figure 1B). S. epidermidis, which is phylogenetically closely related to S. aureus, exhibited little reactivity with anti-S. aureus. Further, B. atrophaeus and E. coli (members of Firmicutes and Proteobacteria, respectively) showed little or no reactivity ( Figure 1B). To determine the specificity of anti-S. aureus, we performed flow cytometric analyses. Consistent with the ELISA results, anti-S. aureus, reacted with BM1006 and SA003 but not with the control IgG ( Figure 1C). Weak reactivity was detected when S. epidermidis was incubated with anti-S. aureus, but reactivity with B. atrophaeus or E. coli was undetectable (Figure 1C). SDS-PAGE and western blot analyses revealed that molecules with a broad range of molecular masses that are specifically expressed by S. aureus but not by S. epidermidis, B. atrophaeus, or E. coli were recognized by anti-S. aureus ( Figure 1D). Some bands detected by both anti-S. aureus and control IgG may be due to the reactivity of natural IgG antibodies or non-specificity ( Figure 1D

Growth in vitro of S. aureus is inhibited by anti-S. aureus
We found that the growth of S. aureus BM1006 and SA003 was delayed in the presence of anti-S. aureus but was undetectable when control IgG was added to the cultures (Figure 2A). Bacterial growth in vitro was inhibited by a relatively high concentration of anti-S. aureus (1000 µg/mL), although the overall effect was concentration-dependent ( Figure 2A). Moreover, anti-S. aureus did not detectably inhibit the growth of E. coli (Figure 2A).
These results indicate that the antigen-specific binding of anti-S. aureus (not nonspecific binding, such as the interaction between Protein A on the surface of S. aureus and the Fc region of IgG antibodies) may be required for inhibiting bacterial growth in vitro. However, the growth of S. aureus plateaued when cultured overnight with or without the anti-S. aureus (Figure 2A), indicating that the inhibitory effect of anti-S. aureus was not sustained during overnight culture. Therefore, we performed a study using an ELISA to determine the change of anti-S. aureus concentrations during an overnight incubation of the culture with 1000 μg/mL anti-S. aureus. The concentration of total IgG antibodies remained constant, although the concentration of total IgG in PBS ( Figure 2B) was 721.5 ± 181.2 μg/mL (not 1000 μg/mL). This may be explained by the use of different assays. For example, the nonspecific BCA protein assay was used basically to measure the purified IgG. Further, a lower concentration was calculated when anti-S. aureus was suspended in TS medium (395.1 ± 37.5 μg/mL 0 h after culture), suggesting that the bovine IgG ELISA system was affected by the solvent. Nevertheless, there were no significant differences in the concentrations of total IgG antibodies in the culture broth during the overnight culture. In contrast, the S. aureus-specific IgG titer of the culture broth gradually decreased during the 24 h culture when the assay used ELISA plates immobilized with killed or live S. aureus ( Figure 2B). Inhibition was not detected when the anti-S. aureus used for overnight culture was reused for the in vitro bacterial culture study, consistent with the undetectable antibody titer specific for live S. aureus 24 h after the culture ( Figure 2C). The anti-S. aureus is a polyclonal antibody that likely recognized different epitopes. Therefore, these results indicate that a small amount of antibodies in the anti-S. aureus preparation reacted with S. aureus and inhibited bacterial growth in vitro.

Antibody-mediated growth inhibition of S. aureus is not due to bacterial agglutination
Considering that CFU do not indicate the bacterial number, the actual number of S. aureus may be different from the CFU number in cases where one colony does not necessarily arise from one bacterium upon bacterial agglutination. To dispel this concern, we directly measured the diameter of S. aureus during the in vitro culture using a microbial particle counter, which allows us to investigate the size and autofluorescence intensity of particles in the sample. We found that the number of S. aureus whose size and autofluorescence intensity increased was gradually elevated when cultured in the presence of both anti-S. aureus and control IgG ( Figure 3A and Additional file 2).
In contrast, such altered S. aureus was rarely observed when cultured without any supplementation ( Figure 3A and Additional file 2). We next performed an SEM analysis to directly observe the morphological characteristics of S. aureus in the in vitro culture. Consistent with the well-known feature of S. aureus, we observed the typical structure of S. aureus in the form of a grape-like cluster when cultured with neither anti-S. aureus nor control IgG ( Figure 3B). Importantly, the morphological characteristics (not the bacterial number) seemed to remain unchanged, although S. aureus was cultured in the presence of anti-S. aureus or control IgG ( Figure 3B). Because the fragment crystallizable (Fc) region of IgG antibodies binds to protein A on the surface of S. aureus [26], these results suggest that, regardless of antibody specificities, the IgG antibodies that associate with Protein A may alter bacterial features (other than growth or agglutination) that can be detected as increased size and autofluorescence intensity, even though the reactivity between Fc region of IgG antibodies and Protein A was almost undetectable in our previous analyses ( Figures 1B-D). Nevertheless, it should be emphasized that these phenomena observed were not due to bacterial agglutination; thus, the inhibition of bacterial growth caused by anti-S. aureus could be mediated in a specific manner of antigen-antibody reaction.

Reactivity of anti-S. aureus to molecules expressed specifically by S. aureus (not commonly expressed by closely related bacteria) are mainly involved in the inhibition of bacterial growth in vitro
Anti-S. aureus slightly reacted with S. epidermidis (Figures 1B and C). Therefore, we hypothesized that antibody recognition of common molecules expressed by Staphylococcus species (e.g., S. aureus and S. epidermidis) was involved in the inhibition of bacterial growth in vitro. We thus incubated anti-S. aureus with S. epidermidis and collected the unbound antibodies by centrifugation to remove reactivity to molecules that are expressed by S. epidermidis. ELISA analysis revealed that anti-S. aureus absorbed with S. epidermidis lost most of its reactivity with S. epidermidis ( Figure 4A). In contrast, although the reactivity to S. aureus was slightly lower when compared with the original anti-S. aureus, it was detected after absorption with S. epidermidis ( Figure 4A). Moreover, when anti-S. aureus was absorbed with S. epidermidis,  In contrast, residual killed and live S. aureus-specific IgG titers gradually decreased during the overnight culture. C The anti-S. aureus that was used in the overnight culture of S. aureus did not inhibit bacterial growth when reused. Three separate experiments were performed, and the data represent mean ± standard error of the mean. The p values calculated using one-way or two-way factorial analyses of variance are # p < 0.05 and ## p < 0.01 or *p < 0.05; **p < 0.01; ***p < 0.001; and ****p < 0.0001, respectively.

Figure 3 Alteration of bacterial features other than growth or agglutination in S. aureus is caused by bovine IgG antibodies regardless of antigen-antibody reaction.
Bacterial features of S. aureus in culture with anti-S. aureus were determined using a microbial particle counter and a scanning electron microscopy. A Size and autofluorescence intensity that can be observed by the microbial particle counter were increased gradually when S. aureus was cultured with either anti-S. aureus or control IgG whereas those bacterial features were not altered when S. aureus was cultured without any antibody treatment. The analyzer show dots that indicate the detected particles, including mostly bacteria and some debris in the medium. The difference in the color of the dot indicates the difference in the number of particles detected. Bar  little or no effect was observed on the ability of anti-S. aureus to inhibit bacterial growth ( Figure 4B). These results suggest that among the numerous epitopes recognized by anti-S. aureus, its reactivity with specific molecules on S. aureus (not common molecules on both S. aureus and S. epidermidis) are largely responsible for triggering the inhibition of bacterial growth.

Antibody recognition of sortase-A-dependent cell-wall-associated proteins is not associated with the capacity of anti-S. aureus to inhibit bacterial growth in vitro
Cell-wall-associated proteins that bind peptidoglycans produced by S. aureus are involved in pathogenicity and are therefore considered targets of an S. aureus vaccine [27]. Hence, we asked whether the recognition of anti-S. aureus to cell-wall-associated proteins inhibited bacterial growth in vitro. We used JE2, which is a plasmid-cured derivative of strain LAC, originally isolated from a human, and its deletion mutant that lacks the gene (JE2ΔSrtA) encoding sortase A because sortase A acts on many cell-wall-associated proteins to link them to peptidoglycans [28]. We found that the reactivities of anti-S. aureus with JE2 and JE2ΔSrtA were comparable ( Figure 5A). Further, there was no significant difference between the reactivity with BM1006 or SA003 ( Figure 1B). However, we did not exclude the possibility that certain sortase-A-dependent cell-wall-associated proteins were recognized by anti-S. aureus. Moreover, the SDS-PAGE and western blot data suggest that anti-S. aureus reacted with multiple surface molecules, such as membrane proteins that are not substrates of sortase, cell wall polysaccharides, or both ( Figure 1D). Therefore, we addressed the possibility of sortase-A-independent growth inhibition induced by anti-S. aureus as indicated by the results of the in vitro bacterial culture study using JE2 and JE2ΔSrtA. We found that the growth of JE2 and JE2ΔSrtA was inhibited when they were cultured in the presence of anti-S. aureus ( Figure 5B), indicating that the inhibition of bacterial growth by anti-S. aureus was sortase-A-independent. Together, our results provide new insights into devising strategies for developing an S. aureus vaccine that induces signals that inhibit bacterial growth or blocks absorption of nutrients that are essential for bacterial growth by vaccine-induced antibodies specific for surface molecules that have not been previously identified.

Discussion
Increasing the efficacy of mastitis vaccines is a global challenge in the field of dairy science [29]. We focused on investigating the inhibitory effect on bacterial growth by specific bovine IgG antibodies produced by vaccination of a Holstein calf with S. aureus killed with formalin. S. aureus is frequently found in the nose, respiratory tract, and skin of animals, including humans and livestock [30][31][32]. S. aureus is not always pathogenic, but occasionally causes infectious diseases, such as pneumonia, infective endocarditis, sepsis, osteomyelitis, and mastitis [33]. The molecular mechanisms of S. aureus infection is under investigation; however, several molecules expressed by S. aureus are involved in pathogenesis [34]. For example, exotoxin (staphylococcal enterotoxins and toxic shock syndrome toxin-1) [35,36], binding molecules (clumping factors and fibronectin binding proteins) [37], and receptors (iron acquisition factors and manganese uptake receptors) [38,39] are virulence factors involved in the pathogenesis. Therefore, numerous studies of virulence molecules were performed to develop an effective vaccine to protect humans as well as dairy cattle from infectious diseases caused by S. aureus. However, despite numerous human clinical trials, most studies have failed to demonstrate the efficacy of S. aureus vaccines [33]. Moreover, efforts to develop S. aureus vaccines for dairy cattle lag behind those of humans [33]. Antibiotics have been widely used in dairy cattle to treat subclinical mastitis caused by S. aureus; however, this may increase the risk of selecting for antibiotic-resistant bacteria, including MRSA [40]. Therefore, a vaccine strategy, which is independent of antibiotic resistance, to control the growth of S. aureus is important to prevent the distribution of antibiotic-resistant S. aureus. Nevertheless, it is noteworthy that the bovine mastitis vaccine STARTVAC ® elicits the production of S. aureus-specific bovine IgG antibodies that inhibit the formation of biofilms and facilitates opsonization [7][8][9]. We show here, for the first time to our knowledge, that S. aureus-specific bovine IgG antibodies directly inhibited the growth of S. aureus without supplementation (e.g., complement) in an in vitro culture system. The effects of anti-S. aureus were similar among strains, because the growth of JE2, which is a methicillin-resistant S. aureus (MRSA) [41], was equivalently inhibited by treatment with anti-S. aureus compared with BM1006 and SA003, which are non-MRSA strains isolated from the milk of dairy cattle (Figures 2-4).
Gram-positive bacteria like S. aureus develop a thick peptidoglycan layer with cell-wall-associated proteins that are linked to peptidoglycans [42]. The molecular mechanism of the synthesis of a dozen cell-wall-associated proteins in the peptidoglycan layer requires cleavage by sortase A of the peptide bond between Thr and Gly residues in the LPXTG motif, which is a consensus motif present within the C-terminus of sortase substrate proteins [27]. Sortase A produces alternative bonds between the carboxyl group of Thr and the amino groups of peptidoglycans [28]. Sortase-A-dependent cell-wallassociated proteins play important roles in infection by S. aureus and mediate adhesion to host cells and evasion of the pathogen of the host's immune system [37]. In addition, such proteins are also involved in absorbing nutrients, such as iron [38]. However, our results acquired using S. aureus JE2ΔSrtA show that the inhibition of bacterial growth by anti-S. aureus was sortase-A-independent ( Figure 5). These findings suggest that potential target molecules, which may be involved in inhibiting bacterial growth through recognition of anti-S. aureus, may not include cell-wall-associated proteins that are substrates of sortase A. The surfaces of gram-positive bacteria such as S. aureus include four major proteins (e.g., membrane proteins, lipoproteins, LPXTG-like proteins, and cellwall-associated proteins) [42][43][44]. Further, cell-wall-associated proteins and extracellular polysaccharides play an important role in host-parasite interactions. Therefore, this information, coupled with our present results, led us to hypothesize that membrane proteins, which are not sortase substrates, polysaccharides, or both, may stimulate a signaling cascade that inhibits bacterial growth through the recognition of anti-S. aureus.
Using the microbial particle counter, we detected that the size and autofluorescence intensity of S. aureus increased gradually when the bacteria was cultured with IgG antibodies regardless of the specificity. Nevertheless, the morphological and microbiological characteristics of S. aureus cultured with either anti-S. aureus or control IgG were identical to those of bacteria cultured without any supplementation. Thus far, three possibilities (i.e., increase in bacterial size and alteration of bacterial morphology or refractive index) have been considered as factors that increase scattered light emitted by bacteria [45]. However, in SEM analysis no changes (except the reduction of bacterial number) were observed despite the addition of anti-S. aureus to the S. aureus culture. These results suggest that the refractive index of scattered light from S. aureus may be altered when the Fc region of IgG antibodies bind to Protein A on the surface of S. aureus. Another possible hypothesis that causes the increase of autofluorescence intensity may be because of the increase in the amount or quantum yield of Riboflavin, which is a bacteria-derived fluorescence molecule detected by the microbial particle counter used, by the binding of the Fc region of IgG antibodies to Protein A.
Determining the optimal concentration of antibodies required for inhibition of bacterial growth is difficult. For example, we used a concentration of anti-S. aureus that was extremely high (1000 μg/mL), although its effects were concentration-dependent (Figures 2-5). Our results were consistent with the past studies showing the efficacy of egg yolk IgY antibodies specific for S. aureus to inhibit bacterial growth [46,47]. Also, others have shown that monoclonal antibody specific to glucosaminidase, which is an enzyme involved in cell wall digestion during binary fission, can induce the abnormality of bacterial survival [48,49]. Although the optimal concentration of anti-glucosaminidase monoclonal antibody that is sufficient for inhibiting bacterial growth has not yet been addressed, we assumed that it was not high because of high antigenic specificity of monoclonal antibody to single antigen compared with that of polyclonal antibodies to multiple antigens. This may be explained by the use of polyclonal antibodies that include nonspecific antibodies. However, our goal is to identify molecules that can be used as novel antigens to develop a mastitis vaccine and not to utilize aureus with S. epidermidis, the S. aureus-specific antibody titer was slightly decreased compared with that of the original anti-S. aureus. In contrast, little reactivity of anti-S. aureus with S. epidermidis was detected after absorption. B Anti-S. aureus absorbed with S. epidermidis did not inhibit the growth of S. aureus. Three separate experiments were performed, and the data represent the mean ± standard error of the mean. The p values calculated using one-way or two-way factorial analyses of variance are # p < 0.05 or ***p < 0.001 and ****p < 0.0001, respectively. the anti-S. aureus as passive prophylaxis. Therefore, our current efforts focus on identifying target molecules that stimulate inhibitory signals to suppress bacterial growth by binding vaccine-induced antibodies. Western blot analysis detected several candidate molecules recognized by anti-S. aureus ( Figure 1D), which must be identified. Further, advances in microbiological research led to the creation of a transposon mutant library of S. aureus, which may facilitate efforts to identify target molecules, such as those not related to sortase substrates, cell wall polysaccharides, or both, in our anti-S. aureus in vitro culture system. Taken together, our findings have significant potential to further improve mastitis vaccines through antibody-mediated growth control of target bacteria. The involvement of antibody recognition of sortase-A-dependent cell-wall-associated proteins in the growth inhibition of S. aureus was addressed using S. aureus strains JE2 and JE2ΔSrtA. A ELISA analyses revealed that anti-S. aureus reacted equally with JE2 and JE2ΔSrtA. B The growth of JE2 and JE2ΔSrtA was delayed when they were cultured with anti-S. aureus. Three separate experiments were performed, and the data represent the mean ± standard error of the mean. The p values calculated using one-way or two-way factorial analyses of variance are # p < 0.05 or *p < 0.05; **p < 0.01 and ***p < 0.001, respectively.