The morphology and metabolic changes of Actinobacillus pleuropneumoniae during its growth as a biofilm

Table 3 Identification of H-NS binding sites^a in the promoter regions of differentially expressed genes (BF compared with PK)

Functional class	Target genes^b	Transcription change^c
Transcription, Translation	rpsP-rimM-trmD-rplS, psG-fusA-tuf, rpsT, rpsL	Down
Transcription, Translation	rimP-nusA-infB	Up
Energy metabolism	ppc, pta-ackA, nrfABCD, atpEFHAGDC, glpX, fumC	Down
Energy metabolism	fdhE, fdhC, can	Up
Carbohydrate metabolism	tpiA, mdh, tal	Down
Carbohydrate metabolism	lld	Up
Amino acids	mmsB, cysK, metE, ackA	Down
Amino acids	marC, filY	Up
Membrane transport	afuA	Down
Membrane transport	fhuCD, fliY, sitCD	Up
Signal transduction	hfq, htpG	Up
Cofactors and Vitamins	fabB, pntAB	Down
Cofactors and Vitamins	folB, ribF, ribDEBA, hemA, folC, lipA	Up
Lipid metabolism	fabB	Down
Lipid metabolism	glpQ	Up
Folding, sorting and degradation	hfq, secG	Down
Folding, sorting and degradation	rppH, htpG	Up
Cellular community	cyaA, secG, hfq	Down
Cellular community	fis, cpdA, APPSER1_RS00145	Up
Replication and repair	rnhA, nfo	Up
Nucleotide metabolism	ushA, cpdB, pyrE, purL-APPSER1_RS09210, cyaA	Down
Nucleotide metabolism	cpdA, rsml	Up

^aThe H-NS motif used for searching was RATAWH.
^bTarget genes were predicted from the MEME-FIMO online tool, p-value < 0.001.
^cTranscriptional changes of target genes were derived from differentially expressed genes in RNA-Seq data, Down, down-regulated, log₂FC ≤ -1, FDR < 0.05; Up, up-regulated, log₂FC ≥ 1, FDR < 0.05.

ISSN: 1297-9716