Figure 3

Transcriptome levels and gene expression difference analysis of PK, BF and Δpga. A Heatmap of differentially expressed genes from all comparison groups (PK, BF and Δpga) after RNA-seq analysis. The horizontal line represents genes, while each column represents a sample. Each treatment group consisted of three independent biological replicates. Red: genes with increased expression levels. Blue: genes with decreased expression levels. B Principal component analysis of gene expressions from all comparison groups (PK, BF and Δpga) after RNA-seq analysis. Different colors represent different groups. C The log₂ fold change values of qRT-PCR showing the transcriptional changes of 11 differentially expressed genes in BF compared with PK group from RNA-seq analysis. D Pearson coefficient correlation analysis between qRT-PCR and RNA-seq analysis. X-axis: log₂ value from qRT-PCR; Y-axis: log₂ value from RNA-seq.