Figure 1

Growth curve and morphological structures of A. pleuropneumoniae biofilm and planktonic bacteria. A Growth curves represented by bacterial culture absorbance at OD_600nm and colony forming units (CFU) of A. pleuropneumoniae planktonic bacteria (PK) with shaking at 37 °C. B Growth curves of biofilm cells (BF) shown by CFU of the upper and adhesion layers of the biofilm cultures grown statically in 12-well plates (bar graphs). The total CFU of both upper and adhesion layers of the biofilm is shown (black line). Biomass of the biofilms were determined by crystal violet (CV) staining of the adhesion layer (purple line). C Morphology of PK and BF grown cells under an optical microscope (OM; Gram staining), transmission electron microscope (TEM) and scanning electron microscope (SEM). The magnification of the image is shown. For growth curves and CV staining of biofilms, data are shown as means ± SD from three independent replicates.