Figure 6

Quantitation of mucus-producing cells in duodenum, mid-jejunum, ileum and colon of pigs at 3 days, 3 weeks and 3 months of age [cryosections fixed on Blotting-Nylon 66 membranes (type B, positive); PAS]. A Representative pictures of the PAS-stained cryosections fixed on Blotting-Nylon 66 membranes (type B, positive) for the different intestinal regions of each age-group. B Number of PAS⁺ cells per villus, crypt and Brunner’s glands under one villus and crypt in all parts of interest, in the different intestinal areas of three age groups. Individual mean values ± SD of three animals per age group are plotted. Significant differences in between age groups (two-way ANOVA with pairwise comparisons) are presented as *p < 0.05, **p < 0.01, ***p < 0.001 or ****p < 0.0001. Scale bar: 200 µm.