Figure 1

Identification of CDV H protein–host protein interaction network. A Identification of host proteins interacting with CDV H protein by mass spectrometry. 293 T cells were transfected with Flag vector, and Flag-CDV H for 24 h. Cell lysates were incubated with anti-Flag antibody overnight at 4 °C, and then protein A/G plus agarose was added for 4 h at 4 °C. The beads were washed and eluted with NP-40 buffer. A small fraction of eluents was subjected to Western blot with anti-Flag antibody, and a band of Flag-CDV H protein was visualized. The remaining eluents were analyzed by mass spectrum, and 89 specific proteins were identified to interact with CDV H protein in the Venn diagram. B GO analysis of CDV H-associated host proteins. Data show the top 10 GO terms enriched in molecular functions, biological processes, and cellular component functional groups. C KEGG analysis of CDV H-associated host proteins. D CDV H protein–host protein interaction map generated using Cytoscape software. Dark gray lines correspond to high-confidence PPI curated in the publicly available STRING database. Nodes with multiple colors indicate host proteins involved in multiple biological functions.