Proof of concept for multiplex detection of antibodies against Chlamydia species in chicken serum using a bead-based suspension array with peptides as antigens

Table 4 Summarized results of field sera tested with the suspension array

Peptides		Number of positive normalized MFI values				Positive sera per Chlamydia species^a
Name	Description	Per peptide	Per farm type
Name	Description	Per peptide	Free range layers	Conventional layers	Broilers
pCga001	Cga_08DC63_OmpA_326-345	0	22	28	0	20
pCga002	Cga_08-1274/3_IncA_297-326	8
pCga003	Cga_08DC63_IncA_303-326	14
pCga008	C. gal G47_OmpA	0
pCga009	C. gal F725_OmpA	0
pCga010	C. gal G47_IncA	15
pCga011	C. gal F725_IncA	13
pCps001	Cps_02DC15_OmpA_158-181	0	2	8	0	7
pCps002	Cps_02DC15_IncA_329-352	2
pCps009	Cps_02DC15_OmpA_250-265	0
pCps010	Cps_02DC15_OmpA_329-352	3
pCps013	Cps_02DC15_PmpD_1053-92	0
pCps014	Cps_02DC15_IncA_321-360	2
pCps015	Cps_02DC15_CT618_105-134	3
pCps016	Cps_02DC15_CT618_189-228	0
pCab001	Cab_S26/3_OmpA_89-104	1	0	6	0	6
pCab002	Cab_S26/3_OmpA_153-176	0
pCab003	Cab_S26/3_OmpA_158-173	0
pCab004	Cab_S26/3_PmpD_1064-1087	0
pCab005	Cab_S26/3_IncA_324-353	5
pCav001	Cav_10DC88_IncA_305-328	1	4	0	7	6
pCav002	Cav_10DC88_IncA_299-328	5
pCav003	Cav_10DC88_IncA_325-348	1
pCav004	Cav_10DC88_IncA_319-348	4
	Seropositive sera per farm type^a:		12	18	3

Results are presented for signals that in the suspension array exceeded the serum-specific cutoffs. Sera were from 120 chickens from three types of farm with resp. free range layers, conventional layers, and broilers.
^aOf the 33 seropositive sera, 28 are seroreactive toward peptides of 1 Chlamydia species, 4 and 1 serum are seroreactive toward peptides of respectively 2 and 3 species.

ISSN: 1297-9716