Proof of concept for multiplex detection of antibodies against Chlamydia species in chicken serum using a bead-based suspension array with peptides as antigens

Table 3 Seroreactivity and sequence identity of ***C. gallinacea*-derived peptides**

C. gallinacea-derived peptides	Blast results of peptide (query) against C. gallinacea NL_G47 (subject)	Signal in infected animals
		Mice	Chickens
		C.gal. 08–1274/3 (08DC63)	C.gal. NL_G47
pCga001_Cga_08DC63_OmpA_326-345	Query 1 NPSFLGSADAQA––––TLVDSVQI 20	y	n
	Sbjct 328..T.S.G.VP.TGGTGSV..V... 351
pCga002_Cga_08-1274/3_IncA_297-326	Query 1 SEAATSTSTPEGETSETKEGEEDSSVVEFD 30	y	freq
	Sbjct 297..........................I... 326
pCga003_Cga_08DC63_IncA_303-326	Query 1 TSTPEGETSETKEGEEDSSVVEFD 24	y	y
	Sbjct 303....................I... 326
pCga008_C.galG47_OmpA	Query 1 NPTFSGGAVPQTGGTGSVVDVVQI 24	n	incons
	Sbjct 328........................ 351
pCga009_C.galF725_OmpA	Query 1 NPSFLGEANAQA----KLVDSVQI 20	n	n
	Sbjct 328..T.S.G.VP.TGGTGSV..V... 351
pCga010_C.galG47_IncA	Query 1 SEAATSTSTPEGETSETKEGEEDSSVIEFD 30	y	y
	Sbjct 297.............................. 326
pCga011_C.galF725_IncA	Query 1 SEAATSTSTPEGETSETKEGEEDSSVIDFD 30	y	y
	Sbjct 297...........................E.. 326

For all C. gallinacea-derived peptides, the seroreactivity in the suspension array with sera from mice and chickens is given. Alignments show the sequences of the used peptides and corresponding sequences of strain NL_G47. Identical amino acids are indicated with dots.
y yes, n no, freq frequently (in all chickens, but signals are late, low, or transient), inconsis inconsistently (in 3 out of 8 chickens).

ISSN: 1297-9716