Figure 9

H2BE effectively inhibits apoptosis in the ER stress pathway. Marc-145 cells were transfected with si-H2BE or NC-si, incubated for 36 h, pretreated with the ER stress inhibitor 4-PBA for 1 h, infected with PEDV at an MOI of 1.0, and incubated for 24 h. A The expression levels of GRP78, CHOP, cleaved caspase-3 and PEDV N protein were measured by Western blotting. B The intensity represents GPR78, CHOP and PEDV N protein levels normalized to the level of β-actin, and the intensity represents the level of cleaved caspase-3 normalized to that of caspase-3. C Marc-145 cells were transfected with si-H2BE or NC-si, infected with PEDV at an MOI of 1.0 and incubated for 24 h, and stained with Annexin V and PI. The results are representative of three independent experiments. The data are presented as the mean ± SD, n = 3, (*P < 0.05; **P < 0.01).