Figure 8

H2BE inhibits PEDV-induced apoptosis. Marc-145 cells were transfected with H2BE-Flag or an empty vector, infected with PEDV at an MOI of 1.0 and incubated for 12 h and 24 h. A The expression levels of Bcl-2, Bax, caspase-9, and caspase-3 were measured by Western blotting. Marc-145 cells were transfected with H2BE-Flag or empty vector for 36 h and infected with PEDV. Cells were harvested 24 h after PEDV infection. B The expression levels of caspase-3 and cleaved caspase-3 protein were measured by Western blotting. C The intensity represents Bax normalized to Bcl-2. D The intensity represents the level of cleaved caspase-9 normalized to that of caspase-9. E The intensity represents the level cleaved caspase-3 normalized to that of caspase-3. Marc-145 cells were transfected with H2BE-Flag or an empty vector, incubated for 36 h and pretreated with Z-VAD-FMK 1 h before PEDV infection. F The expression levels of PEDV N protein were measured by Western blotting. Marc-145 cells were transfected with si-H2BE or NC-si, infected with PEDV at an MOI of 1.0 and then incubated for 12 h and 24 h. G The expression levels of Bcl-2, Bax, caspase-9 and caspase-3 were measured by Western blotting. Marc-145 cells were transfected with si-H2BE or NC-si, incubated for 36 h, and infected with PEDV. Cells were harvested 24 h after PEDV infection. H The expression levels of caspase-3 and cleaved caspase-3 protein were measured by Western blotting. I The intensity represents Bax normalized to Bcl-2. J The intensity represents the level of cleaved caspase-9 normalized to that of caspase-9. K The intensity represents the level of cleaved caspase-3 normalized to that of caspase-3. The results are representative of three independent experiments. The data are presented as the mean ± SD, n = 3, (*P < 0.05; **P < 0.01).