Figure 6

Overexpression of H2BE alleviates PEDV-induced ER stress. Marc-145 cells were transfected with H2BE-Flag or empty vector and then infected with PEDV at an MOI of 1.0 for 12 h and 24 h. A The protein expression levels of GRP78 and CHOP were measured by Western blotting. B The relative intensity represents GRP78 and CHOP protein levels normalized to β-actin. C The protein expression levels of PERK and eIF2 were measured by Western blotting. D The relative intensity represents phosphorylated PERK protein levels normalized to β-actin. The relative intensity represents phosphorylated eIF2 protein levels normalized to β-actin. E The protein expression levels of IRE1 and JNK were measured by Western blotting. F The relative intensity represents phosphorylated IRE1 protein levels normalized to that of β-actin. The relative intensity represents phosphorylated JNK protein levels normalized to that of β-actin. G The expression levels of ATF6 and cleaved ATF6 protein were measured by Western blotting. The results are representative of three independent experiments. The data are presented as the mean ± SD, n = 3, (*P < 0.05; **P < 0.01).