Figure 5

H2BE has no effect on the expression of interferon. Marc-145 cells were transfected with H2BE-Flag or an empty vector and incubated for 36 h, infected with PEDV at an MOI of 1.0 and harvested 12 h and 24 h later. A ISG15 mRNA was detected and analysed by qPCR. B IFN-β mRNA was detected and analysed by qPCR. Marc-145 cells were transfected with H2BE-Flag (si-H2BE) or an empty vector (NC-si), incubated for 36 h and then infected with PEDV at an MOI of 1.0. Then, the cells were placed in 4 ℃ adsorption for 1 h. Marc-145 cells were transfected with H2BE-Flag (si-H2BE) or an empty vector (NC-si), incubated for 36 h and then infected with PEDV at an MOI of 1.0. Then, the cells were placed at 4 ℃ for an hour and endocytosed at 37 ℃ for an hour. C, D PEDV N mRNA was measured by qPCR. (E) LC3II mRNA was detected and analysed by qPCR. F The expression levels of LC3II protein were measured by Western blotting. β-Actin was assessed as an internal control. The results are representative of three independent experiments. The data are presented as the mean ± SD, n = 3, (*P < 0.05; **P < 0.01).