Figure 4

Knockdown of H2BE inhibits PEDV replication. A Three H2BE-targeting siRNAs and nontargeting control siRNA (NC-si) were transfected into Marc-145 cells, and then, the knockdown efficiencies of the three siRNAs were compared by Western blotting. Marc-145 cells were transfected with si-H2BE or NC-si and then infected with PEDV at an MOI of 1.0 and harvested 12 h and 24 h later. B The PEDV N protein level was measured by Western blotting. C PEDV N mRNA level was measured by qPCR. β-Actin was used as the internal control. D The intensity represents the PEDV N protein level normalized to that of β-actin. E PEDV titres in the culture supernatants were determined using the TCID₅₀ method. The results are representative of three independent experiments. The data are presented as the mean ± SD, n = 3, (*P < 0.05; ** < 0.01). Marc-145 cells were transfected with si-H2BE or NC-si and then infected with PEDV at an MOI of 1.0. F Cells were fixed and incubated with mouse anti-PEDV Nsp9 polyclonal sera (1:500) 24 h post-infection. Immunofluorescence assays were used to further observe intracellular propagation of PEDV. G Analysis of H2BE protein fluorescence intensity.