Figure 2

Expression of H2BE after PEDV infection in Marc-145 cells. Marc-145 cells were infected or mock-infected with PEDV at an MOI of 1 and harvested 8 h, 12 h and 24 h later. A H2BE mRNA expression levels were detected and analysed by qPCR. β-Actin was used as the internal control. B H2BE protein expression levels were measured by Western blotting. C The intensity represents the H2BE protein level normalized to that of β-actin. Marc-145 cells were infected with PEDV at the indicated MOI (0, 0.5, 1 or 1.5) and harvested 24 h later. D H2BE mRNA expression levels were measured by qPCR. E H2BE protein expression levels were measured by Western blotting. F The intensity represents the H2BE protein level normalized to that of β-actin. β-Actin was used as the internal control. Marc-145 cells were infected or mock-infected with PEDV at an MOI of 1 and harvested 24 h later. Cells were fixed and incubated with rabbit anti-H2BE antibody (1:500). G Immunofluorescence assays were performed to further analyse the intracellular expression of H2BE. H Analysis of H2BE protein fluorescence intensity. The results are representative of three independent experiments. The data are presented as the mean ± SD, n = 3, (*P < 0.05; **P < 0.01).