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Figure 10 | Veterinary Research

Figure 10

From: The novel Nsp9-interacting host factor H2BE promotes PEDV replication by inhibiting endoplasmic reticulum stress-mediated apoptosis

Figure 10

Nuclear shuttle function of H2BE is essential for PEDV replication. A Full-length H2BE, C-terminal deletion mutant H2BE-ΔN and N-terminal deletion mutant H2BE-ΔC. Marc-145 cells were separately transfected with an empty vector, H2BE-Flag, H2BE-ΔN-Flag, and H2BE-ΔC-Flag and then infected with PEDV at an MOI of 1.0 and incubated for 12 h. B The expression levels of PEDV N protein were measured by Western blotting. C PEDV N mRNA was measured by q-PCR. β-Actin was used as the internal control. D The intensity represents PEDV N protein levels normalized to the level of β-actin. The results are representative of three independent experiments. The data are presented as the mean ± SD, n = 3, (* P < 0.05; ** P < 0.01). E H2BE deletion mutants with amino acids 1–28 deleted. F The expression levels of PEDV N protein were analysed by Western blotting. H2BE-Flag and H2BE-Δ1-28-Flag were cotransfected with Nsp9-EGFP into Marc-145 cells, which were stained with DAPI (blue) and antibodies against Flag (red). G The subcellular localization of H2BE-WT and H2BE-Δ1-28 with Nsp9-EGFP.

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