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Figure 6 | Veterinary Research

Figure 6

From: Fowl adenovirus serotype 4 enters leghorn male hepatocellular cells via the clathrin-mediated endocytosis pathway

Figure 6

FAdV-4 entry depends on macropinocytosis and requires PI3K. A CLSM analysis of F-actin (red), FITC-dextran (green) and DAPI (blue) in FAdV-4-infected LMH cells pretreated with EIPA. B Western blot analysis after the entry of FAdV-4 into EIPA- or wortmannin-treated cells. β-Actin was used as the internal control. B qPCR analysis after the entry of FAdV-4 into EIPA- or wortmannin-treated cells. C CLSM analysis of F-actin (red), anti-FAdV-4 fibre 2 (green) and DAPI (blue) in FAdV-4-infected LMH cells pretreated with EIPA or wortmannin. Bars, 20 μm. *, P < 0.05; **, P < 0.01.

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