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Figure 5 | Veterinary Research

Figure 5

From: Fowl adenovirus serotype 4 enters leghorn male hepatocellular cells via the clathrin-mediated endocytosis pathway

Figure 5

Caveolae-mediated endocytosis is not the pathway by which FAdV-4 enters LMH cells. A Western blot analysis of the protein expression of caveolin-1 after the entry of FAdV-4 in cells transfected with caveolin-1 siRNA and Neg.siRNA. B qPCR analysis after the entry of FAdV-4 into cells transfected with caveolin-1 siRNA and Neg.siRNA. C Western blot analysis after the entry of FAdV-4 into genistein-treated cells. β-Actin was used as the internal control. D qPCR analysis after the entry of FAdV-4 into genistein-treated cells. E CLSM analysis of F-actin (red), anti-FAdV-4 fibre 2 (green) and DAPI (blue) in FAdV-4-infected LMH cells pretreated with genistein. Bars, 20 μm. *, P < 0.05; **, P < 0.01.

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Veterinary Research

ISSN: 1297-9716