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Table 1 PCR primers used in the study

From: Orf virus induces complete autophagy to promote viral replication via inhibition of AKT/mTOR and activation of the ERK1/2/mTOR signalling pathway in OFTu cells

Primers

Sequence (5′-3′)

PCMV-C-Flag-Rheb-F

CCAAGCTTCTGCAGGAATTCATGCCGCAGTCCAAGTCC

PCMV-C-Flag-Rheb-R

TTGTAATCTCTAGACTCGAGCATCACCGAGCAGGAAGACT

PCMV-C-Flag-RhebQ64L-F

AGCTGGGCTGGATGAATATTCCATCTTTCCTCAG

PCMV-C-Flag-RhebQ64L-R

ATTCATCCAGCCCAGCTGTGTCCACAAGTTGA

PCMV-Myc-N-Akt-F

GGCCATGGAGGCCCGAATTCGGATGAACGACGTGGCCGT

PCMV-Myc-N-Akt-R

GCGGCCGCGGTACCTCAGGCCGTGCCGCTG

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Veterinary Research

ISSN: 1297-9716