Figure 6
ORFV titres are positively correlated with the level of autophagy. A OFTu cells were treated with 30 μM Rapa, 5 mM 3-MA, 1 nM Baf A1 or normal medium and then infected with ORFV-CL18. At 60 hpi, the cell culture supernatant was harvested, and the virus titre was by TCID50 assay. The results are expressed as the mean ± SD (n = 3). One-way ANOVA was used to determine significance. Differences were considered statistically significant when P < 0.05. (**P < 0.01, **** P < 0.0001; one-way ANOVA). B OFTu cells were treated with 30 μM Rapa, 5 mM 3-MA, 1 nM Baf A1 or normal medium and then infected with ORFV-CL18 at different dilutions (1 × 10–2 or 1 × 10–3). At 60 hpi, the cell culture supernatant was harvested, and the virus titre was determined by the plaque assay. Each experiment was performed at least three times. C Plaques on the plate prepared with virus diluted 1 × 10–2 were counted, and the data are expressed as the mean ± SD (n = 3) (*P < 0.05, **** P < 0.0001; one-way ANOVA).