Figure 5

ORFV induces autophagy by activating ERK1/2/mTOR signalling. A OFTu cells were mock infected or infected with ORFV-CL18. At 36, 48 and 60 hpi, the expression levels of p-mTOR (S2448), mTOR, p-TSC2 (S1387), TSC2, p-ERK1/2 (T202/Y204), ERK1/2 and LC3B were measured by Western blotting. GAPDH was used as the loading control. B–E The ratios of p-mTOR/mTOR B, p-TSC2/TSC2 C, p-ERK/ERK D and LC3-II/LC3-I E. These protein bands were quantified by measuring the signal intensity using ImageJ, and the ratios are expressed as the mean ± SD (n = 3) (*P < 0.05, ** P < 0.01, **** P < 0.0001; unpaired t test). F OFTu cells were mock-infected, infected with ORFV-CL18, treated with 20 μM U0126 or treated with 20 μM U0126, infected with ORFV (MOI = 10) and incubated for 60 h. After treatment, the expression levels of p-mTOR (S2448), mTOR, p-ERK1/2 (T202/Y204), ERK1/2 and LC3B were measured by Western blotting. GAPDH was used as the loading control. G The ratios of p-mTOR/mTOR, p-ERK/ERK and LC3-II/LC3-I, which are expressed as the mean ± SD (n = 3) (*P < 0.05, ** P < 0.01, *** P < 0.001; one-way ANOVA).