Table 1 Primers used in this study
Name | Sequence(5’→3’) | Purpose |
|---|---|---|
pCAGGS-C-HA-F | CATCATTTTGGCAAAGCCACCATGTCTAACAAAAAACCAGGA | Gene cloning |
pCAGGS-C-HA-R | TTGGCAGAGGGAAAACTAAGCGTAATCTGGAACATCGTATGGGTACCGACGTTTCGCCTTCCG | |
pCAGGS-prM-HA-F | CATCATTTTGGCAAAGCCACCATGGGGGGGAGTTGCTCTTGG | |
pCAGGS-prM-HA-R | TTGGCAGAGGGAAAACTAAGCGTAATCTGGAACATCGTATGGGTAGCTGTACGCTGGGGCAAT | |
pCAGGS-NS1-HA-F | CATCATTTTGGCAAAGCCACCATGGGCCTGAATGCAAGGGAC | |
pCAGGS-NS1-HA-R | TTGGCAGAGGGAAAACTAAGCGTAATCTGGAACATCGTATGGGTAAGCCATGACCTTTGATTT | |
pCAGGS-NS2A-HA-F | CATCATTTTGGCAAAGCCACCATGTTTCAAGGGGGTGGCATG | |
pCAGGS-NS2A-HA-R | TTGGCAGAGGGAAAACTAAGCGTAATCTGGAACATCGTATGGGTATCTCCGTGTCACTGGCTT | |
pCAGGS-NS2B-HA-F | CATCATTTTGGCAAAGCCACCATGGGGTGGCCAGTCAGTGAG | |
pCAGGS-NS2B-HA-R | TTGGCAGAGGGAAAACTAAGCGTAATCTGGAACATCGTATGGGTATCGTTGTTTTGCCTTAGT | |
pCAGGS-NS3-HA-F | CATCATTTTGGCAAAGCCACCATGGGAGGAGTCATCTGGGAT | |
pCAGGS-NS3-HA-R | TTGGCAGAGGGAAAACTAAGCGTAATCTGGAACATCGTATGGGTATCTCTTTCCACTCGCAAA | |
pCAGGS-NS2B3-HA-F | CATCATTTTGGCAAAGCCACCATGGGGTGGCCAGTCAGTGAG | |
pCAGGS-NS2B3-HA-R | TTGGCAGAGGGAAAACTAAGCGTAATCTGGAACATCGTATGGGTATCTCTTTCCACTCGCAAA | |
pCAGGS-NS4A-HA-F | CATCATTTTGGCAAAGCCACCATGTCAGCGATAGGGATCCTT | |
pCAGGS-NS4A-HA-R | TTGGCAGAGGGAAAACTAAGCGTAATCTGGAACATCGTATGGGTATCTCTGTCTCTCTGGTTC | |
pCAGGS-NS4B-HA-F | CATCATTTTGGCAAAGCCACCATGTCACAGACGGATAGTCAC | |
pCAGGS-NS4B-HA-R | TTGGCAGAGGGAAAACTAAGCGTAATCTGGAACATCGTATGGGTATCGACGCAAGGTCCCTGC | |
pCAGGS-NS5-HA-F | CATCATTTTGGCAAAGCCACCATGGGAGGAGGAACTGGCAGA | |
pCAGGS-NS5-HA-R | TTGGCAGAGGGAAAACTAAGCGTAATCTGGAACATCGTATGGGTACAAAACACCTTCACTCCA | |
TMUV-E-F | AATGGCTGTGGCTTGTTTGG | qPCR |
TMUV-E-R | GGGCGTTATCACGAATCTA | |
GAPDH-F | GGAGCGAGATCCCTCCAAAAT | |
GAPDH-R | GGCTGTTGTCATACTTCTCATGG |