Figure 4

Antibody responses and cytokine gene expression in comparison with various adjuvants. Analysis of the titre of total IgG (A), cytokine IFN-γ (B) and IL-4 (C) gene expression. Piglets were intramuscularly immunized twice (week 0 and 2 weeks). Sera were collected at week 0, 2 weeks and 4 weeks, and whole blood was collected at 2 weeks after immunization. S/P ratio = (Sample OD value − Negative OD value)/(Positive OD value − Negative OD value). Data are presented as the mean ± SD. Different superscript letters ^{(a, b, c)} indicate significant differences (p < 0.05) between treatment groups at the same time point based on Duncan’s test.