Figure 4

CUR ameliorated neuronal apoptosis in PRV-infected BV2 cells. PC-12 cells containing neuronal properties were treated with the supernatants of different phenotypes of BV2 cells for 24 h. A The viability of PC-12 cells (n = 4). B LDH activity in PC-12 cells (n = 3). C MDA levels in PC-12 cells (n = 3). D Representative Western blot analysis of apoptotic/antiapoptotic proteins; β-actin was used as the loading control (n = 3). E Relative protein levels of cleaved caspase-3. F Relative protein levels of Bax. G Relative protein levels of Bcl-2. All experiments were performed in parallel. The results are presented as the mean ± SD. Statistical significance was determined using one-way ANOVA followed by an LSD post hoc test for multiple comparisons among the groups. *P < 0.05, **P < 0.01, ***P < 0.001, and NS, not significant.