Figure 2

Curcumin (CUR) suppresses proinflammatory cytokine release and promotes anti-inflammatory cytokine release in PRV-infected BV2 cells. A BV2 cells were treated with different concentrations of CUR for 24 h, and cell viability was determined by the CCK-8 assay. BV2 cells were infected with/without 1.66 × 10⁶ TCID₅₀ PRV for 24 h, followed by dimethyl sulfoxide (DMSO) or 20 μM CUR treatment for 6, 12, and 24 h. The levels of NO (B) in the supernatant were detected using the Griess method; the levels of TNF-α (C) and IL-6 (D), IL-4 (E), and IL-10 (F) were detected using ELISA; and the levels of intracellular ROS (G, H) were detected by adding the fluorescent probe DCFH-DA. Green fluorescence indicates the presence of ROS in BV2 cells; scale bar = 200 μm. All experiments were performed in parallel. The results are presented as the mean ± SD of four biological replicates (n = 4). Statistical significance was determined using one-way ANOVA followed by an LSD post hoc test for multiple comparisons among the groups. *P < 0.05, **P < 0.01, ***P < 0.001, and NS, not significant.