Figure 1
Pseudorabies virus (PRV)-infected BV2 cells show increased production of inflammatory markers. BV2 cells were infected with 1.66 × 106 TCID50, 1.66 × 105 TCID50, 1.66 × 104 TCID50, and 1.66 × 103 TCID50 PRV for 24 h. The levels of NO (A) in the supernatant were detected using the Griess method, the levels of TNF-α (B) and IL-6 (C) were detected using ELISA, and the levels of intracellular ROS (D, E) were detected by adding a fluorescent probe DCFH-DA. Furthermore, the levels of NO (F), TNF-α (G), IL-6 (H), and ROS (I, J) were measured at 6, 12, and 24 h after infection with 1.66 × 106 TCID50 PRV. Green fluorescence indicates the presence of ROS in BV2 cells; scale bar = 200 μm. All experiments were performed in parallel. The results are presented as the mean ± standard deviation (SD) of four biological replicates (n = 4). Statistical significance was determined using one-way analysis of variance (ANOVA) followed by a least significant difference (LSD) post hoc test for multiple comparisons among the groups. *P < 0.05, **P < 0.01, ***P < 0.001, and NS, not significant.