Figure 6

Average percentage of Mycoplasma hyopneumoniae-specific cytokine-producing T cell subsets by maternally derived antibody levels. A-C Circulating M. hyopneumoniae-specific cytokine-producing CD4⁺CD8⁺ T cells in pigs on farm A and B; D, E cytokine-producing CD4⁺CD8⁻ and CD4⁻CD8⁺ T cells in blood of pigs on farm B (farm A is not shown, as there were no significant cytokine responses in CD4⁺CD8⁻ and CD4⁻CD8⁺ T cells seen in animals from farm A). Pigs were vaccinated against M. hyopneumoniae at 16 days of age on both farms. On each farm in 20 piglets M. hyopneumoniae-specific maternally derived antibodies (MDA) were present and in 5 piglets MDA were absent (present: S / P ratio > 0.40; absent: S / P ratio ≤ 0.40) at two-days of age.