Figure 5

Circulating Mycoplasma hyopneumoniae-specific cytokine-producing T cell subsets in blood of pigs on farm B. A CD4⁺CD8⁻ TNF-α; B CD4⁻CD8⁺ IFN-γ; C CD4⁻CD8⁺ IL-17A. The horizontal grey line indicates the median. On both farms pigs were vaccinated against M. hyopneumoniae at 16 days of age. PBMCs were stimulated with M. hyopneumoniae J strain bacterin and T cell phenotype and cytokine production were assessed by flow cytometry. **, P ≤ 0.01; ***, P ≤ 0.001 between the sampling moments. Red dot: M. hyopneumoniae PCR positive animal.