Figure 4

Circulating Mycoplasma hyopneumoniae-specific cytokine-producing CD4⁺CD8⁺ T cells in blood of pigs on both farms. A TNF-α; B IFN-γ, C TNF-α IFN-γ; D IL-17A. The horizontal grey line indicates the median. On both farms, pigs were vaccinated against M. hyopneumoniae at 16 days of age. PBMCs were stimulated with M. hyopneumoniae J strain bacterin and T cell phenotype and cytokine production were assessed by flow cytometry on a monthly basis. **, P ≤ 0.01; ***, P ≤ 0.001 between the sampling moments. Red dot: M. hyopneumoniae PCR positive animal.