Figure 7
Cell types identified in the chicken enteroid lamina propria. Expression of A innate immune function related genes, B glial cell, E mesenchymal cell, J smooth muscle genes and K intestinal development gene sets in freshly isolated villi (0 h), 1, 3, 4 and 7 day chicken enteroids was compared by RNA sequencing analysis. Heat maps show the mean relative expression levels (log2 counts per million reads) of a range of mammalian gene-sets. RNA sequencing data is representative of 3 independent experiments, each comprising of 2 technical replicates, each containing 3 embryos. C–D, F–I Confocal images of chicken enteroids stained for lamina propria cell population markers (green) and counterstained with DAPI (blue) and Phalloidin (red). Enteroids at 2 days of culture stained for (C, D) HuC/HuD (green, glial cells), (F, G) vimentin (green, mesenchymal cells), (H, I) βIII-tubulin (green, mesenchymal cells). Scale bar: 20 µm. Images are representative of data from at least 3 independent cultures each containing 2–3 embryos.