Figure 5

Single gene analyses related to stem cell homeostasis and epithelial cell differentiation. Expression of A stem cell markers, B Wnt signalling pathway genes, and C intestinal epithelial cell differentiation related genes at 0 h and enteroids at 1, 3, 4 and 7 days of culture were analysed by RNA sequencing. Heat maps show the mean relative expression levels (log2 counts per million reads) of a range of mammalian gene-sets. RNA sequencing data is representative of 3 independent experiments, each comprising of 2 technical replicates, each containing 3 embryos.