Figure 1
Analysis of LPS profiles from S. Typhimurium with various OAg lengths. A Identification of gene replacement mutants by silver-stained SDS-PAGE. The lower band was unsubstituted lipid A-core, whereas the upper cluster of bands showed LPS with different lengths of OAg. Lane 1, 2 and 3 represents ST03 (ΔwzzST), ST04 (ΔwzzST::wzzECO2) and ATCC14028, respectively. ST03 (ΔwzzST) was assigned into short OAg length group (< 15 RUs), ST04(ΔwzzST::wzzECO2) was assigned into intermediate OAg length group (15–20 RUs), and the RUs of WT strain ATCC14028 was assigned into long OAg length group (20–35 RUs). B Western blotting detection against anti-Salmonella O4 serum. C LPS profiles of attenuated S. Typhimurium with different OAg lengths. D Identification of panel C by Western blotting. Lane 4, 5, 6 represents ST06 (ATCC14028ΔwzzSTΔcrpΔcya), ST07 (ATCC14028ΔwzzST::wzzECO2ΔcrpΔcya) and ST05 (ATCC14028ΔcrpΔcya), respectively. E LPS profiles of E. coli O2 synthesized in S. Typhimurium ST08 (ATCC14028ΔwzzSTΔcrpΔcyaΔasdΔrfbP) and ST09 (ATCC14028ΔwzzST::wzzECO2ΔcrpΔcyaΔasdΔrfbP). Lane 7, 8, 9 represents ATCC14028, E. coli O2, O2-ST08, and O2-ST09, respectively. F Western blotting of LPS with rabbit antiserum against serotype O2 E. coli.