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Figure 6 | Veterinary Research

Figure 6

From: Porcine epidemic diarrhea virus activates PERK-ROS axis to benefit its replication in Vero E6 cells

Figure 6

Inhibition of ERO1α by EN460 reduced cytosolic ROS and viral replication in PEDV-infected Vero E6 cells. Vero E6 cells were infected with PEDV (MOI = 0.001) with or without EN460 (5 μM). Dimethylsulfoxide (DMSO) was used as solvent control. A The effects of ERO1α inhibition on CHOP, ERO1α, and PEDV N expression were shown by Western blotting with β-actin used as loading control. B Ratios of ERO1α to β-actin. C Ratios of CHOP to β-actin. D Ratios of N to β-actin. E Cells collected at 36 hpi were measured for changes in cytosolic ROS level by flow cytometry after incubation with 10 μM DCFH-DA for 30 min at 37 °C. F The changes in cytosolic ROS levels were evaluated according to the mean value of DCFH-DA fluorescence intensity and expressed as fold changes between mock- and treated-cells. The effects of ERO1α inhibition by shRNA on viral replication, shown as viral RNA copy numbers measured by RT-qPCR (G) and virus titer (H). The bar charts in B to D, F to H represent mean ± SD of three independent experiments. ns, not significant; *, P < 0.05; **, P < 0.01.

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